Anti-GRP94抗体(ab52031)
Key features and details
- Goat polyclonal to GRP94
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GRP94抗体
参阅全部 GRP94 一抗 -
描述
山羊多克隆抗体to GRP94 -
宿主
Goat -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan
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免疫原
Synthetic peptide:
C-KEGVKFDESEKTKE
, corresponding to internal sequence amino acids 593-606 of Human GRP94 -
阳性对照
- WB: NIH-3T3, HEK 293, A549, HeLa and U-2OS cell lysates; IHC: Human liver and uterus tissue. ICC/IF: A431 and HeLa cells. Flow Cyt (intra): A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52031于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 3 - 5 µg/ml.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB | (1) |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
IHC-P
Use a concentration of 3 - 5 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. -
序列相似性
Belongs to the heat shock protein 90 family. -
细胞定位
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 100009456 Rabbit
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: O18750 Rabbit
see all -
别名
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
图片
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Western blot - Anti-GRP94 antibody (ab52031)Lanes 1-3 : Anti-GRP94 antibody (ab52031) at 0.1 µg/ml
Lane 4 : Anti-GRP94 antibody (ab52031) at 0.3 µg/ml
Lane 1 : U2OS cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A594 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Flow Cytometry (Intracellular) - Anti-GRP94 antibody (ab52031)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-GRP94 antibody (ab52031)Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + U-2OS cell lysate
Predicted band size: 92 kDa -
Western blot - Anti-GRP94 antibody (ab52031)Anti-GRP94 antibody (ab52031) at 0.1 µg/ml + NIH-3T3 cell lysate
Developed using the ECL technique.
Predicted band size: 92 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
数据表及文件
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Datasheet download
文献 (1)
ab52031 被引用在 1 文献中.
- Salibe-Filho W et al. Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension. PLoS One 15:e0242960 (2020). PubMed: 33270690
