AB52031

Anti-GRP94抗体

Name: GRP94抗体 (ab52031)| Abcam中文官网
Brand: Abcam Limited
SKU: AB52031
Price: 4845 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-GRP94 antibody

(1 Review)

(3 Publications)

Goat Polyclonal GRP94 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HSP90B1 aa 550-650.

查看别名

GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB52031)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB52031)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB52031)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow Cytometry (Intracellular) - Anti-GRP94 antibody (AB52031)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GRP94 antibody (AB52031)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supplier Data

Western blot - Anti-GRP94 antibody (AB52031)

All lanes:

Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL

All lanes:

NIH-3T3 cell lysate

Predicted band size: 92 kDa

true

Supplier Data

Western blot - Anti-GRP94 antibody (AB52031)

Lanes 1 - 3:

Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL

Lane 4:

Western blot - Anti-GRP94 antibody (ab52031) at 0.3 µg/mL

Lane 1:

U2OS cell lysate at 35 µg

Lane 2:

HEK293 cell lysate at 35 µg

Lane 3:

HeLa cell lysate at 35 µg

Lane 4:

A594 cell lysate at 35 µg

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-GRP94 antibody (AB52031)

All lanes:

Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL

All lanes:

U-2OS cell lysate

Predicted band size: 92 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

免疫原

Synthetic Peptide within Human HSP90B1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

P14625

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Syrian hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.

Biological function summary

GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.

Pathways

GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.

GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed : 18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed : 32272059).

See full target information HSP90B1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed41059571

2025

AI662270/GRP94 axis couples the unfolded protein response to mitochondrial dynamics during acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Suling Ding,Wen Liu,Zhiwei Zhang,Xiyang Yang,Dili Sun,Jianfu Zhu,Xiaowei Zhu,Shijun Wang,Mengshi Xie,Hongyu Shi,Junbo Ge,Xiangdong Yang

International journal of medical sciences 18:2814-2827 PubMed34220309

2021

Abnormal proinflammatory and stressor environmental with increased the regulatory cellular IGF-1/PAPP-A/STC and Wnt-1/β-Catenin canonical pathway in placenta of women with Chronic venous Disease during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martínez,Miguel A Saez,Miguel A Álvarez-Mon,Ana M Gómez-Lahoz,Coral Bravo,Juan A De León Luis,Felipe Sainz,Santiago Coca,Ángel Asúnsolo,Jorge Monserrat,Luis G Guijarro,Melchor Álvarez-Mon,Julia Bujan,Natalio García-Honduvilla

PloS one 15:e0242960 PubMed33270690

2020

Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

William Salibe-Filho,Thaís L S Araujo,Everton G Melo,Luiza B C T Coimbra,Monica S Lapa,Milena M P Acencio,Orival Freitas-Filho,Vera Luiza Capelozzi,Lisete Ribeiro Teixeira,Caio J C S Fernandes,Fabio Biscegli Jatene,Francisco R M Laurindo,Mario Terra-Filho

View all publications

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Anti-GRP94抗体

Anti-GRP94 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB52031)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB52031)

Flow Cytometry (Intracellular) - Anti-GRP94 antibody (AB52031)

Western blot - Anti-GRP94 antibody (AB52031)

Western blot - Anti-GRP94 antibody (AB52031)

Western blot - Anti-GRP94 antibody (AB52031)