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重组RabMAb

重组Anti-GRP78 BiP抗体[EPR4041(2)] (ab108615)

Reviews (3) Submit a question References (72)
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Flow Cytometry (Intracellular) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
  • Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4041(2)] to GRP78 BiP
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-GRP78 BiP抗体[EPR4041(2)]
    参阅全部 GRP78 BiP 一抗

  • 描述

    兔单克隆抗体[EPR4041(2)] to GRP78 BiP

  • 宿主

    Rabbit

  • 特异性

    Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-Pmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, NIH/3T3, LnCaP, MCF7, 293T, U-87 MG, THP-1, MDA-MB-231 and HepG2 whole cell lysate (ab7900); Human liver lysates, Mouse heart, lung, liver and testis lysates; Rat liver lysates. Huvec and RAW 264.7 cells treated with tunicamycin. IHC-P: Human breast carcinoma Tissue Flow Cyt (intra): HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4041(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108615于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/200.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples.  Please see images for recommended treatment conditions and positive controls.
IHC-P (1)
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/50 - 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
说明
Flow Cyt (Intra)
1/200.
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples.  Please see images for recommended treatment conditions and positive controls.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/50 - 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

  • 疾病相关

    Autoantigen in rheumatoid arthritis.

  • 序列相似性

    Belongs to the heat shock protein 70 family.

  • 细胞定位

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 78 kDa glucose regulated protein antibody
    • 78 kDa glucose-regulated protein antibody
    • AL022860 antibody
    • AU019543 antibody
    • BIP antibody
    • D2Wsu141e antibody
    • D2Wsu17e antibody
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
    • Epididymis secretory sperm binding protein Li 89n antibody
    • FLJ26106 antibody
    • Glucose Regulated Protein 78 antibody
    • Glucose Regulated Protein 78kDa antibody
    • GRP 78 antibody
    • GRP-78 antibody
    • GRP78 antibody
    • GRP78_HUMAN antibody
    • Heat shock 70 kDa protein 5 antibody
    • Heat Shock 70kDa Protein 5 antibody
    • Heat shock protein family A (Hsp70) member 5 antibody
    • HEL S 89n antibody
    • Hsce70 antibody
    • HSPA 5 antibody
    • HSPA5 antibody
    • Immunoglobulin Heavy Chain Binding Protein antibody
    • Immunoglobulin heavy chain-binding protein antibody
    • mBiP antibody
    • MIF2 antibody
    • Sez7 antibody
    see all

图片

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml
    tunicamycin for 12 hours whole cell lysates
    Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml
    tunicamycin for 48 hours whole cell lysates
    Lane 5 : MEF (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 
    Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

    Exposure time: 
    Lanes 1 and 2: 20 seconds
    Lanes 3 and 4: 30 seconds
    Lanes 5 and 6: 5 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

    Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 8 : Human uterus lysates
    Lane 9 : Human liver lysates
    Lane 10 : Mouse heart lysates
    Lane 11 : Mouse lung lysates
    Lane 12 : Mouse testis lysates
    Lane 13 : Mouse liver lysates
    Lane 14 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates
    Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
    Lane 4 : HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates
    Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 6 : Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    All lanes : Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates
    Lane 2 : NIH/3T3 cell lysates
    Lane 3 : LnCaP cell lysates
    Lane 4 : HepG2 cell lysates

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 72 kDa

  • Flow Cytometry (Intracellular) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Flow Cytometry (Intracellular) - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

     

     

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + Raw264.7 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution (purified) + HepG2 at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution (purified) + LnCaP at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 72 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)
    Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615)

文献 (72)

发表研究结果有使用 ab108615？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108615 被引用在 72 文献中.

  • Zhou Y  et al. lncRNA HOTAIRM1 Activated by HOXA4 Drives HUVEC Proliferation Through Direct Interaction with Protein Partner HSPA5. Inflammation 47:421-437 (2024). PubMed: 37898994
  • Lin S  et al. Exosome miR-3184-5p inhibits gastric cancer growth by targeting XBP1 to regulate the AKT, STAT3, and IRE1 signalling pathways. Asia Pac J Clin Oncol 19:e27-e38 (2023). PubMed: 35394683
  • Phan V  et al. Molecular mechanisms in chloroquine-exposed muscle cells elucidated by combined proteomic and microscopic studies. Neuropathol Appl Neurobiol 49:e12877 (2023). PubMed: 36633103
  • Charrin E  et al. Soluble Klotho protects against glomerular injury through regulation of ER stress response. Commun Biol 6:208 (2023). PubMed: 36813870
  • Xing X  et al. Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging. Front Nutr 10:1192758 (2023). PubMed: 37583461
View all Publications for this product

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Western blot abreview for Anti-GRP78 BiP antibody [EPR4041(2)]

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HT-1080 Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
2 µg
Specification
HT-1080 Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Feb 28 2017

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