AB108613

重组Anti-GRP78 BiP抗体[EPR4040(2)]

Anti-GRP78 BiP antibody [EPR4040(2)]

RabMAb
Recombinant
20ul selling size
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(64 Publications)

Rabbit Recombinant Monoclonal GRP78 BiP antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 64 publications.

查看别名

GRP78, HSPA5, Endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using unpurified ab108613 at 1/250

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using unpurified ab108613 at 1/250

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian cancer tissue sections labeling GRP78 BiP with Purified ab108613 at 1 : 500 dilution (1.17 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

Lab

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

Blocking and diluting buffer : 5% NFDM/TBST.

Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938

Exposure time :
Lanes 1 and 2 : 3 seconds
Lanes 3 and 4 : 30 seconds
Lanes 5 and 6 : 5 seconds

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/ml tunicamycin for 12 hours whole cell lysates at 15 µg

Lane 3:

MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 4:

MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates at 15 µg

Lane 5:

MEF (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 6:

MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

false

Lab

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HeLa treated with 2.5 μg/ml tunicamycin for 24h whole cell lysates at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

Lane 4:

HUVEC treated with 10 μg/ml tunicamycin for 48h whole cell lysates at 20 µg

Lane 5:

Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 6:

Raw 264.7 treated with 5 μg/ml tunicamycin for 18h whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Exposure time: 3s

Unknown

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 0.1 µg/mL

All lanes:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

false

Unknown

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

RAW264.7 cell lysate at 10 µg

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Unknown

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

LnCaP cell lysate at 10 µg

Lane 3:

ECV-304 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Lab

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (AB108613)

All lanes:

Western blot - Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 20 µg

Lane 6:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 8:

Human uterus lysates at 20 µg

Lane 9:

Human liver lysates at 20 µg

Lane 10:

Mouse heart lysates at 20 µg

Lane 11:

Mouse lung lysates at 20 µg

Lane 12:

Mouse testis lysates at 20 µg

Lane 13:

Mouse liver lysates at 20 µg

Lane 14:

Rat liver lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Exposure time: 37s

不同偶联物与剂型 (1)

Carrier free

Anti-GRP78 BiP antibody [EPR4040(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4040(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat to 98oC, allow to cool for 10-20 minutes. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GRP78 also known as BiP or HSPA5 is a protein that plays an important role in protein folding and assembly within the endoplasmic reticulum (ER). It weighs approximately 78 kDa hence the name GRP78. This chaperone protein binds to hydrophobic regions of nascent polypeptides to prevent aggregation and misfolding. GRP78 is expressed in high levels in the ER of cells where it monitors cellular stress and aids in maintaining ER homeostasis.

Biological function summary

GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.

Pathways

GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.

GRP78/BiP has been implicated in cancer and neurodegenerative diseases. Overexpression of GRP78 is associated with tumor proliferation and poor prognosis in various cancers as cancer cells heavily rely on its protein-folding capacity to survive. Additionally in neurodegenerative diseases misfolded proteins can accumulate leading GRP78 to attempt counteracting these toxic aggregations highlighting its connection with proteins like tau and amyloid-beta in diseases such as Alzheimer's.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed : 2294010, PubMed : 23769672, PubMed : 23990668, PubMed : 28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed : 1550958, PubMed : 11907036, PubMed : 19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Also binds and inactivates EIF2AK3/PERK in unstressed cells (PubMed : 11907036). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1 and EIF2AK3/PERK, allowing their homodimerization and subsequent activation (PubMed : 11907036). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed : 26045166).. (Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus (PubMed : 15098107, PubMed : 28053106, PubMed : 33432092). Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells (PubMed : 15098107).. (Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed : 20484814, PubMed : 24355926, PubMed : 32487760). Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression (PubMed : 32487760).

See full target information HSPA5

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文献 (64)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:94 PubMed40847353

2025

Inhibition of PRC1 elicits immunogenic cell death by triggering ROS-dependent ER stress in colorectal cancer via the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Lijiang Zhou,Xinyu Zhang,Zheng Li

Medical gas research 15:459-470 PubMed40300881

2025

Magnesium-assisted hydrogen improves isoproterenol-induced heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbao Chen,Ruimin Chen,Lili Yang,Bowen Shen,Yunting Wang,Yongfeng Gao,Rui Tan,Xiaomin Zhao

Cell & bioscience 14:115 PubMed39238058

2024

Targeting stress induction of GRP78 by cardiac glycoside oleandrin dually suppresses cancer and COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Dat P Ha,Woo-Jin Shin,Ze Liu,Michael E Doche,Roy Lau,Nektaria Maria Leli,Crystal S Conn,Mariangela Russo,Annalisa Lorenzato,Constantinos Koumenis,Min Yu,Shannon M Mumenthaler,Amy S Lee

Histology and histopathology 39:1685-1693 PubMed39037126

2024

Oridonin alleviates inflammation and endoplasmic reticulum stress in pediatric pneumonia via regulating the SIRT1-mediated Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weijuan Han,Chen Qian,Peipei Fu,Junmei Xu

eLife 13: PubMed38963323

2024

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Juan Wang,Xiao-Jing Di,Pei-Pei Zhang,Xi Chen,Marnie P Williams,Dong-Yun Han,Raad Nashmi,Brandon J Henderson,Fraser J Moss,Ting-Wei Mu

Frontiers in pharmacology 14:1265551 PubMed38026991

2023

ShenQiWan ameliorates renal injury in type 2 diabetic mice by modulating mitochondrial fusion and endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xiong,Jing Feng,Yumeng Liu,Jiapeng Liu,Liang Fu,Qian Wang,Xia Li,Shuyu Li

BMC ophthalmology 23:286 PubMed37353739

2023

Knockout of PERK protects rat Müller glial cells against OGD-induced endoplasmic reticulum stress-related apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Wang,Xinxing Zhu,Guangqian Huang,Lili Wu,Zhiyong Meng,Yuyu Wu

Proceedings of the National Academy of Sciences of the United States of America 120:e2211087120 PubMed37216524

2023

Germline mutation causes a multisystem chaperonopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Erger,Rajindra P Aryal,Björn Reusch,Yasuyuki Matsumoto,Robert Meyer,Junwei Zeng,Cordula Knopp,Maxence Noel,Lukas Muerner,Andrea Wenzel,Stefan Kohl,Nikolai Tschernoster,Gunter Rappl,Isabelle Rouvet,Jutta Schröder-Braunstein,Felix S Seibert,Holger Thiele,Martin G Häusler,Lutz T Weber,Maike Büttner-Herold,Miriam Elbracht,Sandra F Cummings,Janine Altmüller,Sandra Habbig,Richard D Cummings,Bodo B Beck

Biological & pharmaceutical bulletin 46:979-986 PubMed37183023

2023

Forsythiaside A Alleviates Lipopolysaccharide-Induced Acute Liver Injury through Inhibiting Endoplasmic Reticulum Stress and NLRP3 Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Nan Fu,Shu-Chang Liu,Yi Chen,Jie Zhao,Ning Lu,Tao Ma

Frontiers in molecular neuroscience 16:1175364 PubMed37152434

2023

Endoplasmic reticulum stress and ubiquitin-proteasome system impairment in natural scrapie.

Applications

Unspecified application

Species

Unspecified reactive species