重组Anti-Grp75/MOT抗体[EPR5326(2)] (ab129201)
![Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.cn/ps/products/129/ab129201/Images/ab129201-143260-anti-grp75-mot-antibody-epr53262-western-blot.jpg "Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5326(2)] to Grp75/MOT
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Grp75/MOT抗体[EPR5326(2)]
参阅全部 Grp75/MOT 一抗 -
描述
兔单克隆抗体[EPR5326(2)] to Grp75/MOT -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Grp75/MOT aa 650-750 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, Jurkat, K562, and MCF-7 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 4.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5326(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129201于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa).
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 74 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
序列相似性
Belongs to the heat shock protein 70 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Omim: 600548 Human
- SwissProt: P38646 Human
- SwissProt: P38647 Mouse
- SwissProt: P48721 Rat
- Unigene: 184233 Human
see all -
别名
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
图片
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Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.cn/ps/products/129/ab129201/Images/ab129201-143259-anti-grp75-mot-antibody-epr53262.jpg)
OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) -
![Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)](https://www.abcam.cn/ps/products/129/ab129201/Images/ab129201-3-benefits-of-recombinant-antibodies.png)
Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab129201 被引用在 3 文献中.
- Cai G et al. Rosmarinic Acid Inhibits Mitochondrial Damage by Alleviating Unfolded Protein Response. Front Pharmacol 13:859978 (2022). PubMed: 35652041
- Bhattacharyya R et al. Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes. Cell Rep 35:109134 (2021). PubMed: 34010653
- Nadeem A et al. Beta-sheet-specific interactions with heat shock proteins define a mechanism of delayed tumor cell death in response to HAMLET. J Mol Biol 431:2612-2627 (2019). PubMed: 31082436