Anti-Growth Hormone抗体[GH-1] (ab9821)
![Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)](https://www.abcam.cn/ps/products/9/ab9821/Images/ab9821-17482-anti-growth-hormone-antibody-gh-1-western-blot.jpg "Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)")
Key features and details
- Mouse monoclonal [GH-1] to Growth Hormone
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Growth Hormone抗体[GH-1]
参阅全部 Growth Hormone 一抗 -
描述
小鼠单克隆抗体[GH-1] to Growth Hormone -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Growth Hormone.
Database link: P01241 -
表位
Ab9821 recognizes a different epitope than GH-2 (ab9822). -
常规说明
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
GH-1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 22-24 kDa.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 22-24 kDa. |
靶标
-
功能
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. -
疾病相关
Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
序列相似性
Belongs to the somatotropin/prolactin family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 2688 Human
- Omim: 139250 Human
- SwissProt: P01241 Human
- Unigene: 655229 Human
-
别名
- gH antibody
- GH-N antibody
- GH1 antibody
see all
图片
-
Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution
Lane 1 :Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg
Lane 2 :Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22-24 kDa
Exposure time: 2 minutes -
![Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)](https://www.abcam.cn/ps/products/9/ab9821/Images/ab9821_1.gif)
Western blot - Anti-Growth Hormone antibody [GH-1] (ab9821)Western blot of human growth hormone using ab9821 at a concentration of 1 µg/ml.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab9821 被引用在 6 文献中.
- Sleumer B et al. Selective quantification of the 22-kDa isoform of human growth hormone 1 in serum and plasma by immunocapture and LC-MS/MS. Anal Bioanal Chem 414:6187-6200 (2022). PubMed: 35838770
- Bults P et al. Intact protein quantification in biological samples by liquid chromatography - high-resolution mass spectrometry: somatropin in rat plasma. J Chromatogr B Analyt Technol Biomed Life Sci 1144:122079 (2020). PubMed: 32247186
- Liu Y et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int J Mol Med 42:3037-3046 (2018). PubMed: 30272367
- Sun H et al. A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis. Nat Commun 7:10339 (2016). PubMed: 26842342
- Kunert R et al. CHO-recombinant human growth hormone as a protease sensitive reporter protein. Appl Microbiol Biotechnol 84:693-9 (2009). PubMed: 19396439
- Rezaei B et al. Immobilization of specific monoclonal antibody on Au nanoparticles for hGH detection by electrochemical impedance spectroscopy. Biosens Bioelectron 25:395-9 (2009). PubMed: 19692224