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Anti-Growth Cone抗体[2G13] (ab7762)

Reviews (1)Q&A (3)References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (ab7762)

    Key features and details

    • Mouse monoclonal [2G13] to Growth Cone
    • Suitable for: WB, IHC-Fr, ICC/IF, IHC-P
    • Reacts with: Mouse, Rat, Chicken
    • Isotype: IgM

    概述

    • 产品名称

      Anti-Growth Cone抗体[2G13]

    • 描述

      小鼠单克隆抗体[2G13] to Growth Cone

    • 宿主

      Mouse

    • 经测试应用

      适用于: WB, IHC-Fr, ICC/IF, IHC-Pmore details
      不适用于: Flow Cyt

    • 种属反应性

      与反应: Mouse, Rat, Chicken

    • 免疫原

      Tissue, cells or virus corresponding to Chicken Growth Cone. Embryonic chick tectal membranes

    • 常规说明

      This antibody is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.

       

      This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility. This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...

    • 纯度

      Tissue culture supernatant

    • Primary antibody说明

      This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility. This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

    • 克隆

      单克隆

    • 克隆编号

      2G13

    • 骨髓瘤

      NS1

    • 同种型

      IgM

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7762于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration.
    IHC-P
    1/4.

    This product does not require protein digestion pre-treatment sections e.g. trypsin or pronase, and does not require antigen retrieval using heat treatment prior to staining.
    说明
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-P
    1/4.

    This product does not require protein digestion pre-treatment sections e.g. trypsin or pronase, and does not require antigen retrieval using heat treatment prior to staining.
    应用说明
    Is unsuitable for Flow Cyt.

    靶标

    • 相关性

      Growth cones are specialised sensory structures located at the tip of neurites which are involved in the mechanism that enable elongation and navigation of axons during neuronal development.

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (ab7762)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Cone antibody [2G13] (ab7762)

      Immunohistochemical staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using ab7762.

    文献 (4)

    发表研究结果有使用 ab7762？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7762 被引用在 4 文献中.

    • Smith LK  et al. Direct interaction of HIV gp120 with neuronal CXCR4 and CCR5 receptors induces cofilin-actin rod pathology via a cellular prion protein- and NOX-dependent mechanism. PLoS One 16:e0248309 (2021). PubMed: 33705493
    • Kumar H  et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
    • Wülfing C & Günther HS Dendritic cells and macrophages neurally hard-wired in the lymph node. Sci Rep 5:16866 (2015). PubMed: 26581550
    • Stettler O  et al. Monoclonal antibody 2G13, a new axonal growth cone marker. J Neurocytol 28:1035-44 (1999). PubMed: 11054903

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Growth Cone antibody [2G13]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary (E18) hippocampal & spinal neurons)
    Permeabilization
    Yes - 0.2% Triton X100 in PBS, 30'
    Specification
    Primary (E18) hippocampal & spinal neurons
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Alan Howe

    Verified customer

    提交于 Aug 14 2007

    Question

    What starting dilution should I use for IF with this antibody?

    Read More
    Answer

    Thank you for your enquiry. We would recommend a starting dilution of 1:4 for ab7762 in IHC. I hope this information helps. Please do not hesitate to contact us again if you need any further information.

    Read More

    Question

    What is the MW?

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    Answer

    In Western blotting the antibody has been shown to recognize a protein of approximately 48 kDa.

    Read More

    Question

    Is there published literature using this antibody in tissue sections as immunohistochemical stain?

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    Answer

    The only published literature that we are aware of, apart from our antibody data sheet, is:
    Stettler O., Bush, M. S., Kasper, M., Schlosshauer, B. and Gordon-Weeks, P. R. 1999. Monoclonal antibody 2G13, a new axonal growth cone marker. J Neurocytol. 28:1035-1044.

    Read More

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