Anti-groEL抗体[9A1/2] (ab82592)
![Western blot - Anti-groEL antibody [9A1/2] (ab82592)](https://www.abcam.cn/ps/products/82/ab82592/Images/ab82592-97397-anti-groel-antibody-9a1-2-western-blot.jpg "Western blot - Anti-groEL antibody [9A1/2] (ab82592)")
Key features and details
- Mouse monoclonal [9A1/2] to groEL
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG1
概述
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产品名称
Anti-groEL抗体[9A1/2]
参阅全部 groEL 一抗 -
描述
小鼠单克隆抗体[9A1/2] to groEL -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Full length protein corresponding to Escherichia coli groEL. Strain K12
Database link: P0A6F5 -
阳性对照
- E. coli lysate.
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常规说明
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
9A1/2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82592于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
|
IP
Use at an assay dependent concentration. |
靶标
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相关性
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 1037522 Escherichia coli
- SwissProt: P0A6F5 Escherichia coli
- SwissProt: P0A6F6 Escherichia coli
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别名
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
see all
图片
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Western blot - Anti-groEL antibody [9A1/2] (ab82592)All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution
Lane 1 : groEL recombinant E. coli protein
Lane 2 : Hsp60 recombinant human protein (negative control)
Lane 3 : E. coli lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDaThis image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab82592 被引用在 19 文献中.
- Yang W et al. Phosphate (Pi) Transporter PIT1 Induces Pi Starvation in Salmonella-Containing Vacuole in HeLa Cells. Int J Mol Sci 24:N/A (2023). PubMed: 38139044
- Fang J et al. Mycobacterium tuberculosis Rv0790c inhibits the cellular autophagy at its early stage and facilitates mycobacterial survival. Front Cell Infect Microbiol 12:1014897 (2022). PubMed: 36439208
- Yeom J & Groisman EA Low Cytoplasmic Magnesium Increases the Specificity of the Lon and ClpAP Proteases. J Bacteriol 203:e0014321 (2021). PubMed: 33941609
- Bao SH et al. A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA. Cell Rep 36:109413 (2021). PubMed: 34289355
- Schuller M et al. Molecular basis for DarT ADP-ribosylation of a DNA base. Nature 596:597-602 (2021). PubMed: 34408320