Anti-GRK2 (phospho S29)抗体(ab58520)
Key features and details
- Rabbit polyclonal to GRK2 (phospho S29)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GRK2 (phospho S29)抗体
参阅全部 GRK2 一抗 -
描述
兔多克隆抗体to GRK2 (phospho S29) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human GRK2 aa 1-100 (phospho S29).
Database link: P25098 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 80 kDa.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 80 kDa. |
靶标
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功能
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
组织特异性
Expressed in peripheral blood leukocytes. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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数据库链接
- Entrez Gene: 156 Human
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P25098 Human
- SwissProt: P26817 Rat
- Unigene: 83636 Human
- Unigene: 13010 Rat
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别名
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
图片
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Western blot - Anti-GRK2 (phospho S29) antibody (ab58520)All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)
Lane 1 : Jurkat cells treated with EGF
Lane 2 : Jurkat cells treated with EGF with phospho peptide
Predicted band size: 80 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 (phospho S29) antibody (ab58520)This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab58520 被引用在 1 文献中.
- Schreckenberg R et al. Adverse Effects on ß-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function. J Am Heart Assoc 6:N/A (2017). PubMed: 29273639