重组Anti-GRIM19抗体[EPR4471(2)] (ab109017)
![Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.cn/ps/products/109/ab109017/Images/ab109017-393564-anti-grim19-antibody-epr44712-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4471(2)] to GRIM19
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GRIM19抗体[EPR4471(2)]
参阅全部 GRIM19 一抗 -
描述
兔单克隆抗体[EPR4471(2)] to GRIM19 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, Ramos, Jurkat, and 293T cell lysates. IHC-P: Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4471(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109017于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. -
组织特异性
Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. -
疾病相关
Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. -
序列相似性
Belongs to the complex I NDUFA13 subunit family. -
发展阶段
Expressed in numerous fetal tissues. -
细胞定位
Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 51079 Human
- Omim: 609435 Human
- SwissProt: Q9P0J0 Human
- Unigene: 534453 Human
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别名
- 2700054G14Rik antibody
- AU022060 antibody
- B16.6 antibody
see all
图片
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Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFA13 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaLanes 1-3: Merged signal (red and green). Green - ab109017 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109017 Anti-GRIM19 antibody [EPR4471(2)] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab109017 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.cn/ps/products/109/ab109017/Images/ab109017-121590-anti-grim19-antibody-epr44712-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)Immunohistochemical analysis of GRIM19 in paraffin-embedded Human kidney tissue using ab109017 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.cn/ps/products/109/ab109017/Images/ab109017-121591-anti-grim19-antibody-epr44712-western-blot.jpg)
Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa -
![Anti-GRIM19 antibody [EPR4471(2)] (ab109017)](https://www.abcam.cn/ps/products/109/ab109017/Images/ab109017-4-benefits-of-recombinant-antibodies.png)
Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109017 尚未被引用在任何文献中。