Anti-GRIM19抗体[6E1BH7] (ab110240)
![Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.cn/ps/products/110/ab110240/Images/ab110240-393562-anti-grim19-antibody-6e1bh7-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)")
Key features and details
- Mouse monoclonal [6E1BH7] to GRIM19
- Suitable for: WB, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-GRIM19抗体[6E1BH7]
参阅全部 GRIM19 一抗 -
描述
小鼠单克隆抗体[6E1BH7] to GRIM19 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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阳性对照
- WB: HeLa and Jurkat cell lysates; Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria. ICC/IF: Human fibroblasts. Flow Cyt: HeLa cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯化说明
ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
6E1BH7 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110240于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. -
组织特异性
Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis. -
疾病相关
Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. -
序列相似性
Belongs to the complex I NDUFA13 subunit family. -
发展阶段
Expressed in numerous fetal tissues. -
细胞定位
Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 338084 Cow
- Entrez Gene: 51079 Human
- Entrez Gene: 67184 Mouse
- Entrez Gene: 314759 Rat
- Omim: 609435 Human
- SwissProt: Q95KV7 Cow
- SwissProt: Q9P0J0 Human
- SwissProt: Q9ERS2 Mouse
see all -
别名
- 2700054G14Rik antibody
- AU022060 antibody
- B16.6 antibody
see all
图片
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Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFA13 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaLanes 1-3: Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control ab181602 observed at 36 kDa.
ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.cn/ps/products/110/ab110240/Images/ab110240-123414-anti-grim19-antibody-6e1bh7-western-blot.jpg)
Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml
Lane 1 : Human heart mitochondria
Lane 2 : Bovine heart mitochondria
Lane 3 : Rat heart mitochondria
Lane 4 : Mouse heart mitochondria
Predicted band size: 17 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.cn/ps/products/110/ab110240/Images/ab110240-123413-anti-grim19-antibody-6e1bh7-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG. -
![Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)](https://www.abcam.cn/ps/products/110/ab110240/Images/ab110240-123412-anti-grim19-antibody-6e1bh7-flow-cytometry.jpg)
Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab110240 被引用在 59 文献中.
- Mandigers PJJ et al. A novel IBA57 variant is associated with mitochondrial iron-sulfur protein deficiency and necrotizing myelopathy in dogs. Front Genet 14:1190222 (2023). WB ; Dog . PubMed: 37588046
- Hyun M et al. Melatonin protects against cadmium-induced oxidative stress via mitochondrial STAT3 signaling in human prostate stromal cells. Commun Biol 6:157 (2023). PubMed: 36750754
- Cafournet C et al. Novel ELAC2 Mutations in Individuals Presenting with Variably Severe Neurological Disease in the Presence or Absence of Cardiomyopathy. Life (Basel) 13:N/A (2023). PubMed: 36836802
- Webb BD et al. Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency. Elife 12:N/A (2023). PubMed: 36881526
- Ye X et al. Switching ubiquitous and muscle-specific isoforms of mitochondrial respiratory complex IV in skeletal muscle fine-tunes complex IV activity. FASEB J 37:e22891 (2023). PubMed: 36961412