AB110240

Anti-GRIM19 抗体 [6E1BH7]

Name: GRIM19抗体[6E1BH7] (ab110240)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110240
Price: 4598 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-GRIM19 antibody [6E1BH7]

KO Validated
了解详情

(4 Reviews)

(69 Publications)

Mouse Monoclonal GRIM19 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Cow, Rat samples. Cited in 69 publications.

查看别名

GRIM19, CDA016, CGI-39, NDUFA13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, Cell death regulatory protein GRIM-19, Complex I-B16.6, Gene associated with retinoic and interferon-induced mortality 19 protein, NADH-ubiquinone oxidoreductase B16.6 subunit, CI-B16.6, GRIM-19, Gene associated with retinoic and IFN-induced mortality 19 protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (AB110240)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (AB110240)

Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.

Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (AB110240)

Flow Cyt

Unknown

Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (AB110240)

HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Lab

Western blot - Anti-GRIM19 antibody [6E1BH7] (AB110240)

Lanes 1-3 : Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control ab181602 observed at 36 kDa.

ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFA13 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NDUFA13 (GRIM19) knockout HeLa cell line (<a href='/products/cell-lines/human-ndufa13-grim19-knockout-hela-cell-line-ab265863'>ab265863</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 17 kDa,184 kDa,28 kDa,56 kDa,62 kDa,76 kDa

Observed band size: 17 kDa,184 kDa,29 kDa,56 kDa,70 kDa,75 kDa

false

Unknown

Western blot - Anti-GRIM19 antibody [6E1BH7] (AB110240)

All lanes:

Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/mL

Lane 1:

Human heart mitochondria

Lane 2:

Bovine heart mitochondria

Lane 3:

Rat heart mitochondria

Lane 4:

Mouse heart mitochondria

Predicted band size: 17 kDa

false

不同偶联物与剂型 (1)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GRIM19 antibody [6E1BH7]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

6E1BH7

亚型

IgG2b

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Cow, Human

应用

ICC/IF, WB, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化说明

ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GRIM19 also known as NDUFA13 is a small protein with a molecular mass of approximately 16 kDa. It expresses mainly in mitochondria where it forms an integral component of the mitochondrial respiratory chain complex I. GRIM19 plays an important role in the functioning of complex I by facilitating the assembly and stabilization of the complex enabling efficient electron transport and ATP synthesis.

Biological function summary

GRIM19 interacts with several complexes and proteins within the mitochondrial inner membrane. As a part of complex I also known as NADH:ubiquinone oxidoreductase it contributes to the initial step of mitochondrial oxidative phosphorylation. GRIM19 also participates in the regulation of apoptosis and its expression is influenced during cellular stress situations highlighting its significance in cellular survival and energy production.

Pathways

GRIM19 integrates closely with mitochondrial apoptosis and energy metabolism pathways. Its interactions within oxidative phosphorylation place it in concert with both the electron transport chain and the apoptosis regulatory network. GRIM19 shares associations with related proteins such as cytochrome c oxidase and other complex I subunits highlighting its essential contribution to these important biological processes.

GRIM19 demonstrates links to various pathologies including cancer and mitochondrial diseases. Alterations in GRIM19 expression or function have been implicated in the progression of certain cancers such as colorectal and thyroid cancer where GRIM19's usual regulatory role in apoptosis can be disrupted. Additionally its involvement in mitochondrial disease connects to proteins like COX-A1 emphasizing the importance of maintaining functional complexes for cellular health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis (PubMed : 27626371). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed : 27626371). Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes (PubMed : 15753091).

See full target information NDUFA13

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文献 (69)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:5499-5517 PubMed40303326

2025

Nitric oxide-primed engineered extracellular vesicles restore bioenergetics in acute kidney injury via mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Peng,Xiaoniao Chen,Lingling Wu,Jiayi He,Zongjin Li,Quan Hong,Qiang Zhao,Meng Qian,Xu Wang,Wanjun Shen,Tingting Qi,Yiyu Huang,Guangyan Cai,Chuyue Zhang,Xiangmei Chen

The Journal of biological chemistry 301:108433 PubMed40120684

2025

HS remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes.

Applications

Unspecified application

Species

Unspecified reactive species

David A Hanna,Brandon Chen,Yatrik M Shah,Oleh Khalimonchuk,Brian Cunniff,Ruma Banerjee

The Journal of clinical investigation 135: PubMed39786963

2025

Loss of Cpt1a results in elevated glucose-fueled mitochondrial oxidative phosphorylation and defective hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jue Li,Jie Bai,Vincent T Pham,Michihiro Hashimoto,Maiko Sezaki,Qili Shi,Qiushi Jin,Chenhui He,Amy Armstrong,Tian Li,Mingzhe Pan,Shujun Liu,Yu Luan,Hui Zeng,Paul R Andreassen,Gang Huang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2414358 PubMed39746855

2025

ECSIT-X4 is Required for Preventing Pressure Overload-Induced Cardiac Hypertrophy via Regulating Mitochondrial STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Lu,Tingting Tong,Haoliang Sun,Yi Chen,Yongfeng Shao,Pengxi Shi,Linli Que,Li Liu,Guoqing Zhu,Qi Chen,Chuanfu Li,Jiantao Li,Shuo Yang,Yuehua Li

Molecular therapy. Methods & clinical development 32:101372 PubMed39659757

2024

Exogenous expression of ATP8, a mitochondrial encoded protein, from the nucleus .

Applications

Unspecified application

Species

Unspecified reactive species

David V Begelman,Bhavna Dixit,Carly Truong,Christina D King,Mark A Watson,Birgit Schilling,Martin D Brand,Amutha Boominathan

Experimental & molecular medicine 56:2739-2746 PubMed39643607

2024

GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Yeon Jeong,Jin-Sil Park,Jeong Won Choi,Kun Hee Lee,Seung Cheon Yang,Hye Yeon Kang,Sang Hee Cho,Seon-Yeong Lee,A Ram Lee,Youngjae Park,Sung-Hwan Park,Mi-La Cho

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405147 PubMed39488787

2024

HADHA Regulates Respiratory Complex Assembly and Couples FAO and OXPHOS.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoying Qin,Shasha Gong,Ting Liang,Zhenbo Zhang,Jessie Thomas,Janice Deng,Yaguang Liu,Peiqing Hu,Bi Zhu,Shujie Song,Marisol Fernández Ortiz,Yuji Ikeno,Exing Wang,James Lechleiter,Susan T Weintraub,Yidong Bai

Journal of molecular medicine (Berlin, Germany) 102:913-926 PubMed38753040

2024

Loss-of-function mutation in DDX53 associated with hereditary spastic paraplegia-like disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Yuan,Ya Wang,Xiyuan Li,Sheng Zhong,Danyi Zhou,Xianlong Lin,Hezhi Fang,Yanling Yang,Maofeng Wang

NPJ Parkinson's disease 9:120 PubMed37553379

2023

Parkinson's disease neurons exhibit alterations in mitochondrial quality control proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chen,David McDonald,Alasdair Blain,Emily Mossman,Kiera Atkin,Michael F Marusich,Roderick Capaldi,Laura Bone,Anna Smith,Andrew Filby,Daniel Erskine,Oliver Russell,Gavin Hudson,Amy E Vincent,Amy K Reeve

Frontiers in genetics 14:1190222 PubMed37588046

2023

A novel IBA57 variant is associated with mitochondrial iron-sulfur protein deficiency and necrotizing myelopathy in dogs.

Applications

Species

Dog

Paul J J Mandigers,Oliver Stehling,Manon Vos-Loohuis,Frank G Van Steenbeek,Roland Lill,Peter A Leegwater

View all publications

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Anti-GRIM19 抗体 [6E1BH7]