AB198499

重组Anti-GRID2抗体[EPR15422] - N-terminal

Anti-GRID2 antibody [EPR15422] - N-terminal

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal GRID2 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

查看别名

GLURD2, GRID2, GluD2, GluR delta-2 subunit

3 Images

Supplier Data

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution

All lanes:

Human cerebellum lysate (boiled) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 113 kDa

Observed band size: 113 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution

Lane 1:

Mouse brain lysate (boiled) at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate (boiled) at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 113 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 113 kDa

Observed band size: 113 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15422

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The gene symbol 'GRID2' encodes the protein Glutamate Receptor Ionotropic Delta-2 commonly called GRID2. It is part of the ionotropic glutamate receptor family and functions as ion channels in the central nervous system. GRID2 has an approximate mass of 110 kDa and is principally expressed in the cerebellum. This protein receptor plays a role in excitatory synaptic transmission by binding glutamate an essential neurotransmitter. It is not widespread in the body but has critical functions where it is present.

Biological function summary

The GRID2 protein is significant in synaptic plasticity and cerebellar functions by contributing to synapse formation and stability. It is part of the postsynaptic density (PSD) complex in neurons mainly affecting the communication between neuron cells. This protein helps in maintaining proper synaptic connections necessary for motor coordination and learning. The deficit of GRID2 can disrupt these functions affecting synaptic efficacy and neurological behavior.

Pathways

Grid ionotropic receptors like GRID2 play a pivotal role in the glutamatergic pathway and the synaptic signaling pathway. These pathways have strong associations with synaptic transmission and plasticity influencing learning and memory. The relationship of GRID2 with other proteins such as GRIA1 another glutamate receptor highlights its involvement in regulating synaptic strength and communication between neurons.

GRID2 mutations or dysfunctions link to neurological conditions such as spinocerebellar ataxia and autism spectrum disorders. Spinocerebellar ataxia patients show mutations that alter GRID2 function impacting motor coordination. In some autism spectrum disorder cases alterations in GRID2-supported pathways can influence synaptic connectivity which affects cognitive and social functioning. Connections with other proteins like GRIK2 suggest a network where GRID2 dysfunction contributes to these disease symptoms.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Member of the ionotropic glutamate receptor family, which plays a crucial role in synaptic organization and signal transduction in the central nervous system. Although it shares structural features with ionotropic glutamate receptors, does not bind glutamate as a primary ligand (PubMed : 34936451). Promotes synaptogenesis and mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis of cerebellar parallel fiber-Purkinje cell (PF-PC) synapses through the NRX1B-CBLN1-GRID2 triad complex (PubMed : 27418511). In the presence of neurexins and cerebellins, forms cation-selective channels that are proposed to be gated by glycine and D-serine (PubMed : 34936451). However, recent research disputes this ligand-gated cation channel activity (PubMed : 39052831). Cation-selective ion channel activity can be triggered by GRM1 in Purkinje cells (PubMed : 24357660, PubMed : 27276689).

See full target information GRID2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Neuron 112:362-383.e15 PubMed38016472

2023

Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1.

Applications

Unspecified application

Species

Unspecified reactive species

Leon Tejwani,Neal G Ravindra,Changwoo Lee,Yubao Cheng,Billy Nguyen,Kimberly Luttik,Luhan Ni,Shupei Zhang,Logan M Morrison,John Gionco,Yangfei Xiang,Jennifer Yoon,Hannah Ro,Fatema Haidery,Rosalie M Grijalva,Eunwoo Bae,Kristen Kim,Regina T Martuscello,Harry T Orr,Huda Y Zoghbi,Hayley S McLoughlin,Laura P W Ranum,Vikram G Shakkottai,Phyllis L Faust,Siyuan Wang,David van Dijk,Janghoo Lim

Proceedings of the National Academy of Sciences of the United States of America 120:e2219897120 PubMed37459550

2023

Mycoplasma DnaK increases DNA copy number variants in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Benedetti,Giovannino Silvestri,Saman Saadat,Frank Denaro,Olga S Latinovic,Harry Davis,Sumiko Williams,Joseph Bryant,Rudy Ippodrino,Chozha V Rathinam,Robert C Gallo,Davide Zella

Bioscience reports 41: PubMed33969375

2021

Validation of the functions and prognostic values of synapse-associated proteins in lower-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Han Lin,Yong Yang,Chongxian Hou,Yuqing Huang,Liting Zhou,Jiantao Zheng,Guangzhao Lv,Rui Mao,Shanwei Chen,Peihong Xu,Yujun Zhou,Peng Wang,Dong Zhou

Experimental and therapeutic medicine 17:1863-1869 PubMed30783461

2019

Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Huang,Chan Liu,Jia-Rui Li,Ling Zhang,Fu-Chang Huang,Dan Wang,Ya-Jing Luo

View all publications

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重组Anti-GRID2抗体[EPR15422] - N-terminal

Anti-GRID2 antibody [EPR15422] - N-terminal

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (AB198499)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原