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Anti-GREB1抗体(ab72999)

Reviews (2)Q&A (1)References (13)
Western blot - Anti-GREB1 antibody (ab72999)
  • Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab72999)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab72999)

Key features and details

  • Rabbit polyclonal to GREB1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-GREB1抗体
    参阅全部 GREB1 一抗

  • 描述

    兔多克隆抗体to GREB1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab72999于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).
IHC-P
Use a concentration of 1 µg/ml.
说明
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).
IHC-P
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.

  • 组织特异性

    Expressed in proliferating prostatic tissue and prostate cancer.

  • 序列相似性

    Belongs to the GREB1 family.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 5730583K22Rik antibody
      • 9130004E13 antibody
      • AF180470 antibody
      • AU023194 antibody
      • Gene regulated by estrogen in breast cancer protein antibody
      • Gene regulated in breast cancer 1 protein antibody
      • GREB 1 antibody
      • Greb1 antibody
      • GREB1_HUMAN antibody
      • KIAA0575 antibody
      • mKIAA0575 antibody
      • OTTHUMP00000180581 antibody
      • Protein GREB1 antibody
      see all

    图片

    • Western blot - Anti-GREB1 antibody (ab72999)
      Western blot - Anti-GREB1 antibody (ab72999)
      Anti-GREB1 antibody (ab72999) at 1 µg/ml + Human Prostate Tissue Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 216 kDa
      Observed band size: 216 kDa


      Exposure time: 2 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab72999)
      Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab72999)
      ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab72999)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab72999)

      IHC image of GREB1 antibody staining in a section of formalin-fixed paraffin-embedded normal human prostate performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab72999, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    文献 (13)

    发表研究结果有使用 ab72999？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab72999 被引用在 13 文献中.

    • Duijndam B  et al. Physiologically Relevant Estrogen Receptor Alpha Pathway Reporters for Single-Cell Imaging-Based Carcinogenic Hazard Assessment of Estrogenic Compounds. Toxicol Sci 181:187-198 (2021). PubMed: 33769548
    • Tashjian RZ  et al. The effect of estrogen-like compound on rotator cuff tendon healing in a murine model. J Orthop Res N/A:N/A (2021). PubMed: 33533088
    • Kochi M  et al. Oncogenic mutation in RAS-RAF axis leads to increased expression of GREB1, resulting in tumor proliferation in colorectal cancer. Cancer Sci 111:3540-3549 (2020). PubMed: 32629543
    • Haines CN  et al. GREB1 regulates PI3K/Akt signaling to control hormone-sensitive breast cancer proliferation. Carcinogenesis 41:1660-1670 (2020). PubMed: 32894276
    • Yang Y  et al. MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression. Mol Ther Nucleic Acids 22:846-861 (2020). PubMed: 33230480
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    ChIP on chip abreview for Anti-GREB1 antibody

     Good
    Abreviews
    abreview image
    Application
    ChIP on chip
    Detection step
    Microarray
    Sample
    Human Cell lysate - nuclear (Chip-Seq in MCF7 cells)
    Specification
    Chip-Seq in MCF7 cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Chip-seq of GREB1 was performed on asynchronous MCF7 cells crosslinked using formaldehyde. 10ug of DNA was used for each experiment. ChIP DNA was then extracted and amplified using Illumina sequencing adapters and DNA was sequenced.
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 12 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GREB1 antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF7 cells)
    Specification
    MCF7 cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 solution for 5 min
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: .5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 25 2013

    Question

    LOT NUMBER 937692 ORDER NUMBER 1026293 DESCRIPTION OF THE PROBLEM Non-specific band Actural western blot band size different from the predicted SAMPLE MCF-7, SK-BR-3, T47D, MDA-MA-231, MDA-MB-435 cell line PRIMARY ANTIBODY Concentration or dilution 1:300 Diluent buffer 2.5% BSA, PBST, Incubation time overnight Incubation temperature: 4°C DETECTION METHOD Supersignal west pico stable peroxide solution and luminal/enhancer solution(Pierce) were used according to manufacturer’s instructions. POSITIVE AND NEGATIVE CONTROLS USED no ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION NUN buffer containing 0.33 M NaCl, 1.1 M urea, 1% Nonidet P-40, 25 mM HEPES Protease inhibitors: 1 mM DTT, 10 µg/ml leupeptin, 1 mM PMSF, 2 µg/ml aprotinin boling : yes AMOUNT OF PROTEIN LOADED 20 µg/lane ELECTROPHORESIS/GEL CONDITIONS 10% Tris Glycin gel TRANSFER AND BLOCKING CONDITIONS Type of membrane N.C membrane Protein transfer verified with Ponceau S solution Blocking agent and concentration 5% non-fat milk Blocking time 1hr Blocking temperature Room temperature SECONDARY ANTIBODY Pierce Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated (product number: 31458) Isotype: IgG Concentration or dilution 1:7500 Diluent buffer 5% non-fat milk in PBST Incubation time 1hr Incubation temperature: Room temperature washing: PBST HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Change the Primary antibody(Anti-GREB1 antibody) incubation condition from 1hr at RT to overnight at 4°C. ADDITIONAL NOTES I study about the mechanism of cholesterol specially LDLR(Low Density Lipoprotein Receptor) and PCSK9(Proprotein convertase subtilisin/kexin9). Recently, To verify the correlation between estrogen and PCSK9, I treated b-estradiol(sigma) in MCF-7, SK-BR-3, T47D, MDA-MA-231, MDA-MB-435 cell line respectively. Then I identify the protein expression of GREB1 as a marker of estrogen treatment. And GREB1 is very important molecule because it may be a regulator of PCSK9, I expect. So my research purpose is the regulation of GREB1 has an effect on the expression of PCSK9 and further LDLR, cholesterol level.

    Read More
    Answer

    Thank you for your enquiry regardingab72999 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer ishaving problems with this antibody.



    Could you please specify what size are the non-specific bands? Are they lower or higher than the expected size (216 kDa)?





    Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at the bands.



    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

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