AB65771

Anti-GRC5 / PHF2抗体

Anti-GRC5 / PHF2 antibody

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(1 Publication)

Rabbit Polyclonal GRC5 / PHF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PHF2 aa 800 to C-terminus.

查看别名

CENP-35, KIAA0662, PHF2, Lysine-specific demethylase PHF2, GRC5, PHD finger protein 2

1 Images

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Western blot - Anti-GRC5 / PHF2 antibody (AB65771)

All lanes:

Western blot - Anti-GRC5 / PHF2 antibody (ab65771) at 1/1000 dilution

All lanes:

Nuclear extracts of HeLa cells.

Predicted band size: 120 kDa

Observed band size: 130 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human PHF2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75151

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯度

Whole antiserum

存储溶液

Constituents: Whole serum

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GRC5 also known as PHF2 is a protein that functions as a JmjC domain-containing histone demethylase. It specifically demethylates lysine residues on histone H3K9me1 and H3K9me2. This protein has a mass of approximately 125 kDa and expresses widely in human tissues including the liver and brain. The ability of PHF2 to alter chromatin structure makes it a significant regulator of gene expression.

Biological function summary

PHF2 interacts with chromatin remodeling complexes to facilitate transcriptional activation. It is part of the SIRT1-HDAC complex which affects gene expression by modifying the acetylation status of histones and non-histone proteins. By demethylating histones PHF2 plays a role in cellular stress response and metabolic regulation impacting processes like gluconeogenesis and inflammation.

Pathways

PHF2 integrates into transcriptional regulatory networks by modulating epigenetic marks. It participates in the circadian rhythm and metabolic pathways along with related proteins such as CLOCK and ARNTL (BMAL1). These interactions ensure the proper timing of gene expression in response to environmental changes essential for maintaining metabolic homeostasis.

PHF2 relevance is noticeable in metabolic disorders and certain cancers. Aberrant expression or mutations in PHF2 associates with obesity and breast cancer. The protein interacts with pathways involving p53 and SIRT1 which play roles in DNA repair and cell survival pathways implicating PHF2 in the cellular response to oncogenic stress and metabolic imbalance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Lysine demethylase that demethylates both histones and non-histone proteins (PubMed : 20129925, PubMed : 21167174, PubMed : 21532585). Enzymatically inactive by itself, and becomes active following phosphorylation by PKA : forms a complex with ARID5B and mediates demethylation of methylated ARID5B (PubMed : 21532585). Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes (PubMed : 21532585). The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA (PubMed : 21532585). Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters (By similarity).

See full target information PHF2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 27:1052-1066 PubMed31358914

2019

JMJD6 modulates DNA damage response through downregulating H4K16ac independently of its enzymatic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Dawei Huo,Hao Chen,Yiming Cheng,Xin Song,Kai Zhang,Mulin Jun Li,Chenghao Xuan

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Anti-GRC5 / PHF2抗体

Anti-GRC5 / PHF2 antibody

Western blot - Anti-GRC5 / PHF2 antibody (AB65771)