重组Anti-GRB7抗体[EPR6858] (ab129091)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6858] to GRB7
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GRB7抗体[EPR6858]
参阅全部 GRB7 一抗 -
描述
兔单克隆抗体[EPR6858] to GRB7 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- SKBR-3 whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6858 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129091于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
靶标
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功能
Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. -
序列相似性
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
翻译后修饰
Phosphorylated on tyrosine residues. - Information by UniProt
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数据库链接
- Entrez Gene: 2886 Human
- Omim: 601522 Human
- SwissProt: Q14451 Human
- Unigene: 86859 Human
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别名
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
see all
图片
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All lanes : Anti-GRB7 antibody [EPR6858] (ab129091) at 1/1000 dilution
Lanes 1 & 5 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDaExposure time: Lane 1-4: 180 seconds; Lane 5: 7 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The GRB7 expression level in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID 17916906 and 7907978.
This image is derived from purified batch.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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