JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154029

Anti-GRB10 抗体

Anti-GRB10 antibody

4

(3 Reviews)

|

(3 Publications)

Rabbit Polyclonal GRB10 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus.

查看别名

GRBIR, KIAA0207, GRB10, Growth factor receptor-bound protein 10, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR

1 Images
Western blot - Anti-GRB10 antibody (AB154029)
  • WB

Unknown

Western blot - Anti-GRB10 antibody (AB154029)

7.5% SDS PAGE.

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-GRB10 antibody (ab154029) at 1/2000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 67 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Recombinant Fragment Protein within Human GRB10 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13322

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500.00000 - 1/3000.00000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Growth Factor Receptor-Bound Protein 10 often referred to as GRB10 is an adaptor protein with a mass of approximately 66 kDa. This protein interacts with various receptor tyrosine kinases and other intracellular signaling molecules. GRB10 is expressed in many tissues but it shows higher expression in the brain muscle and liver. It plays a significant role as a modulator in cellular processes that involve signal transduction.
Biological function summary

GRB10 interacts with receptor tyrosine kinases such as the insulin receptor and engages in regulating the insulin signaling pathway. It acts as part of a complex that influences cell growth and metabolism. GRB10 binds Via its SH2 domain to activated receptors such as IGF-1R altering downstream signaling and having an impact on growth and metabolic processes.

Pathways

GRB10 is integral to the PI3K-AKT signaling pathway and the mTOR pathway. It negatively regulates these pathways by binding to receptors and altering downstream effects. GRB10 interacts with proteins such as IRS1 and mTORC1 affecting how cells respond to insulin and growth factors. Through these interactions it contributes to cellular metabolism and growth regulation.

Studies show that GRB10 has connections to conditions like cancer and type 2 diabetes. Alterations in GRB10's function or expression can lead to abnormal cellular signaling contributing to oncogenesis due to disrupted growth signals. In type 2 diabetes GRB10 affects insulin signaling pathways which is related to insulin resistance. These disorders show interconnection with proteins like IRS1 where GRB10 modulates pathways influencing disease states.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms : interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
See full target information GRB10
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:21628 PubMed33303821

2020

Saturated fatty acids induce insulin resistance in podocytes through inhibition of IRS1 via activation of both IKKβ and mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Benoit Denhez,Marina Rousseau,Crysta Spino,David-Alexandre Dancosst,Marie-Ève Dumas,Andréanne Guay,Farah Lizotte,Pedro Geraldes

Frontiers in physiology 9:1051 PubMed30131712

2018

Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ge,Shu Zhang,Liang Chen,Min Tang,Lanlan Liu,Mengna Kang,Lu Gao,Shangshang Ma,Yanhua Yang,Peng Lv,Ming Kong,Qin Yao,Fan Feng,Keping Chen

FEBS letters 591:1176-1186 PubMed28295264

2017

The adaptors Grb10 and Grb14 are calmodulin-binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Irene García-Palmero,Noemí Pompas-Veganzones,Eduardo Villalobo,Sophie Gioria,Jacques Haiech,Antonio Villalobo
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB125583

Anti-GRB10 antibody

primary-antibodies

grb10-antibody-ab125583

3

(4 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com