为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Adapters Cytoplasmic

Anti-GRB10抗体(ab125583)

  • Datasheet
  • SDS
Reviews (4)Q&A (4)References (2)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-GRB10 antibody (ab125583)

    Key features and details

    • Rabbit polyclonal to GRB10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    你可能会感兴趣

    Western blot
    Product image
    InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
    蛋白
    Product image
    Recombinant Human GRB10 protein (ab152439)
    二抗
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    查看更多相关产品

    概述

    • 产品名称

      Anti-GRB10抗体
      参阅全部 GRB10 一抗
    • 描述

      兔多克隆抗体to GRB10
    • 宿主

      Rabbit
    • 特异性

      At least three isoforms of GRB10 are known to exist; ab125583 will detect all three isoforms.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide corresponding to 17 amino acids near the C terminus of Human GRB10 (NP_005302).

    • 阳性对照

      • WB: SK-N-SH cell lysate.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human GRB10 protein (ab152439)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab125583于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (2)
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa.
    说明
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa.

    靶标

    • 功能

      Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
    • 组织特异性

      Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
    • 序列相似性

      Belongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • 结构域

      The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
    • 翻译后修饰

      phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
    • 细胞定位

      Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
    • Target information above from: UniProt accession Q13322 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 2887 Human
      • Omim: 601523 Human
      • SwissProt: Q13322 Human
      • Unigene: 164060 Human
      • 别名

        • grb 10 antibody
        • GRB IR antibody
        • grb-10 antibody
        • GRB10 adapter protein antibody
        • GRB10 adaptor protein antibody
        • GRB10 antibody
        • GRB10_HUMAN antibody
        • GRBIR antibody
        • Growth factor receptor bound protein 10 antibody
        • Growth factor receptor-bound protein 10 antibody
        • Insulin receptor binding protein antibody
        • Insulin receptor binding protein GRB IR antibody
        • Insulin receptor-binding protein Grb-IR antibody
        • IRBP antibody
        • KIAA0207 antibody
        • Maternally expressed gene 1 antibody
        • MEG1 antibody
        • RSS antibody
        see all

      图片

      • Western blot - Anti-GRB10 antibody (ab125583)
        Western blot - Anti-GRB10 antibody (ab125583)
        Lane 1 : Anti-GRB10 antibody (ab125583) at 1 µg/ml
        Lane 2 : Anti-GRB10 antibody (ab125583) at 2 µg/ml

        All lanes : SK-N-SH cell lysate

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 67 kDa



        Secondary antibody - anti-rabbit HRP (ab6721)

      实验方案

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab125583?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab125583 被引用在 2 文献中.

      • Zheng P  et al. circHtra1/miR-3960/GRB10 Axis Promotes Neuronal Loss and Immune Deficiency in Traumatic Brain Injury. Oxid Med Cell Longev 2022:3522492 (2022). PubMed: 35571247
      • Stirm L  et al. Maternal whole blood cell miRNA-340 is elevated in gestational diabetes and inversely regulated by glucose and insulin. Sci Rep 8:1366 (2018). Human . PubMed: 29358694

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-8 of 8 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GRB10 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (undifferentiated Human ES cells on MEFs)
      Permeabilization
      Yes - see blocking buffer
      Specification
      undifferentiated Human ES cells on MEFs
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 14 2016

      Western blot abreview for Anti-GRB10 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEPG2)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      HEPG2
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 20 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-GRB10 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEPG2)
      Specification
      HEPG2
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MISS. XXXXXX XXXXXX

      Verified customer

      提交于 Nov 18 2015

      Western blot abreview for Anti-GRB10 antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      1e+006 cells
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic stem cell)
      Specification
      mouse embryonic stem cell
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 21 2014

      Question

      I want proceed with the test of ab125583. Thank you

      Read More

      Abcam community

      Verified customer

      Asked on May 14 2012

      Answer

      DISCOUNT CODE: XXXXXXXXXX
      Expiration date: 13th Sept 2012

      I am very pleased to hear you would like to use ab125583 inPig and send us an image of your results.

      This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration date. To redeem this offer, please submit an Abreview with the image for [PIG] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
      1) Call to place your order and mention the code to our customer service department;
      2) Include the code in your fax order;
      3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

      If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      回复于 May 14 2012

      Question

      I also want to test GRB10 (ab125583). I am sending you the pig sequence:

      Read More

      Abcam community

      Verified customer

      Asked on May 11 2012

      Answer

      Thank you very much for your interest in ab125583

      To our knowledge, ab125583 has not been tested in Pig. Therefore, I can offer a discount off a future purchase if you buy ab125583 now, test it inPig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab125583 in Pig. I will then send a discount code. This code must be issued before purchasing ab125583 so please wait for my reply before ordering.

      2. Purchase ab125583 either by phone, fax, or online (www.abcam.com).

      3. Test it in (PIG).

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab125583 turns out to be unsuitable for (pig), you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      PS: The c-terminal end of Pig and Human is quite homologous so this antibody will cross react.

      Read More

      Abcam Scientific Support

      回复于 May 11 2012

      Question

      you would like to proceed and test ab125583 with pig samples.
      thank you

      Read More

      Abcam community

      Verified customer

      Asked on Apr 30 2012

      Answer

      DISCOUNT CODE: XXXXXXXX
      Expiration date: XXXXXXXX



      I am very pleased to hear you would like to accept our offer and test ab125583 in pig species with WB. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      回复于 Apr 30 2012

      Question

      I am looking for an antibody against GRB10 that recognize the pig. Find below the pig protein sequence.
      thank you

      Read More

      Abcam community

      Verified customer

      Asked on Apr 27 2012

      Answer

      Thank you for contacting us.

      None of our Anti-GRB10 antibody have been tested in pig species, however, one of them, ab125583 is very likely to cross react with this protein in pig species due to sequence homology.

      https://www.abcam.com/grb10-antibody-ab125583.html

      If you are interested in testing ab125583 in pig lysates with WB, I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

      Therefore, I can offer a discount off a future purchase if you buy ab125583 and test it in pig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab125583 with pig samples. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

      2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

      3. Test the antibody.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

      To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Apr 27 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2025 艾博抗(上海)贸易有限公司版权所有