Anti-GRB10 + GRB14抗体(ab317371)
Key features and details
- Rabbit polyclonal to GRB10 + GRB 14
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GRB10 + GRB14抗体 -
描述
兔多克隆抗体to GRB10 + GRB 14 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human GRB10 aa 500-600 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13322 -
阳性对照
- IHC-P: Mouse brain tissue. WB: HepG2 cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 20% Tris buffered saline, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317371于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/300 - 1/5000.
|
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/300 - 1/5000. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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细胞定位
GRB10: Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment. GRB 14: Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Endosome membrane. -
数据库链接
- Entrez Gene: 2887 Human
- Entrez Gene: 2888 Human
- Entrez Gene: 14783 Mouse
- Entrez Gene: 50915 Mouse
- Omim: 601523 Human
- Omim: 601524 Human
- SwissProt: Q13322 Human
- SwissProt: Q14449 Human
see all
图片
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Western blot - Anti-GRB10 + GRB14 Antibody (ab317371)Anti-GRB10 + GRB14 Antibody (ab317371) at 1/300 dilution + HepG2 cell lysate
HepG2 lysate probed with AB317371 at 1:300 dilution and 4degC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37degC.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB10 + GRB14 Antibody (ab317371)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue staining GRB10 + GRB14 using AB317371 at 1:400 overnight at 4degC, DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37degC for 30min.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317371 尚未被引用在任何文献中。