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AB313711

重组Anti-Granzyme K抗体[EPR24601-14] - BSA and Azide free (Detector)

Anti-Granzyme K antibody [EPR24601-14] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Granzyme K antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

TRYP2, GZMK, Granzyme K, GrK, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

1 Images
Sandwich ELISA - Anti-Granzyme K antibody [EPR24601-14] - BSA and Azide free (Detector) (AB313711)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Granzyme K antibody [EPR24601-14] - BSA and Azide free (Detector) (AB313711)

Sandwich ELISA of ab313709 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24601-14

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB314368

Human Granzyme K ELISA Kit

SimpleStep

ELISA - Human Granzyme K ELISA Kit (AB314368)

  • 0
  • 0
Sample Prep & Detection Kits

AB102859

APC/Cy7® Conjugation Kit - Lightning-Link®

Flow Cytometry - APC/Cy7® Conjugation Kit - Lightning-Link® (AB102859)

  • 5
  • 1
Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

  • 0
  • 0
Primary Antibodies

AB290688

Anti-Aquaporin 4 antibody [EPR24281-105] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Aquaporin 4 antibody [EPR24281-105] - BSA and Azide free (Capture) (AB290688)

  • 0
  • 0
Primary Antibodies

AB259549

Anti-C Peptide antibody [EPR22500-5] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-C Peptide antibody [EPR22500-5] - BSA and Azide free (Capture) (AB259549)

  • 0
  • 0
Primary Antibodies

AB259752

Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector) (AB259752)

  • 0
  • 0
Primary Antibodies

AB76225

Anti-VAV1 (phospho Y174) antibody [EP510Y]

20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

  • 5
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme K also known as protein K or 24c3 is a serine protease with a mass of approximately 27 kilodaltons. It belongs to the granzyme family of proteins which are mainly found within the cytotoxic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). These immune cells store granzyme K in their granules which they release to induce apoptosis in target cells. Researchers have identified granzyme K expression in various tissues and immune cells highlighting its widespread role in modulating immune responses.
Biological function summary

Granzyme K serves as a potent mediator in the immune system by inducing apoptosis in target cells. It does not act alone but rather in concert with other granzymes and proteins to effectively execute cell death. Granzyme K can degrade cellular proteins once introduced into a target cell's cytoplasm leading to apoptosis. The protease functions independently and does not require a complex for its activity contributing to the immune system's ability to eliminate infected or transformed cells.

Pathways

The granzyme K protein exists within essential immune regulatory pathways such as the cytotoxic T lymphocyte-induced apoptosis pathway and the natural killer cell-mediated cytotoxicity pathway. Granzyme K acts alongside granzymes A and B to promote programmed cell death and finely tune host immune responses. While granzymes A and B have more defined roles granzyme K complements their activity offering redundancy and ensuring efficient elimination of harmful target cells.

Granzyme K shows significant connections to inflammatory conditions and autoimmune diseases such as rheumatoid arthritis. Elevated granzyme K levels in these diseases may result from dysregulated immune responses leading to excessive tissue damage. In autoimmune disorders granzyme K works in combination with proteins like perforin which facilitates entry of granzymes into the cell to stimulate apoptosis. Understanding granzyme K's role in these conditions may help develop therapeutic approaches to modulate immune activity and minimize tissue damage.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3262682, PubMed : 39814882, PubMed : 39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed : 39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed : 39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed : 39814882, PubMed : 39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed : 24711407, PubMed : 33271118, PubMed : 35704599, PubMed : 39614076, PubMed : 39814882, PubMed : 39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed : 21760880, PubMed : 26936634, PubMed : 35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed : 17308307).
See full target information GZMK
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