JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243879

重组Anti-Granzyme B抗体[BLR022E]

Anti-Granzyme B antibody [BLR022E]

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Recombinant Monoclonal Granzyme B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GZMB.

查看别名

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Granzyme B with ab243879.

Positive staining on human lymph node

Counterstained with Hematoxylin.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B (shaded) in SR (human lymphoma cell line) cells by flow cytometry.

SR cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 106 cells.

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879) or isotype control (unshaded).

Secondary : DyLight® 650-conjugated goat anti-rabbit IgG (ab96886).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Granzyme B with ab243879.

Positive staining on human lung carcinoma

Counterstained with Hematoxylin.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Formalin-fixed, paraffin-embedded human lung carcinoma stained for Granzyme B (red) using ab243879 at 1/250 dilution in immunohistochemical analysis. HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by immunocytochemistry.

Sample : Cell pellet FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right).

Antibody : Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (ab243879).

Secondary : HRP-conjugated goat anti-rabbit IgG.

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • WB

Supplier Data

Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by western blot.

In Western blot, Rabbit anti-COPB2 antibody (ab192924) - used as a Loading Control, lower panel.

Samples prepared using NETN lysis buffer.

Chemiluminescence detection.

All lanes:

Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879) at 1/1000 dilution

Lane 1:

HEK293T (Human Embryonic Kidney cells) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

SR (human lymphoblast cell line) whole cell lysate at 15 µg

Lane 4:

Jurkat (human T-lymphoblastic leukemia cell line) whole cell lysate at 15 µg

Lane 5:

Hep-G2 (Human Hepatoblastoma Cell Line) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 27.7 kDa

Observed band size: 38 kDa

false

Exposure time: 10s

Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)
  • WB

Supplier Data

Western blot - Anti-Granzyme B antibody [BLR022E] (AB243879)

Detection of human Granzyme B by western blot.

In Western blot, Rabbit anti-myc antibody (ab314108) lower panel.

Samples Myc-tagged Granzyme H and B overexpressing cell lysates prepared using NETN lysis buffer.

Chemiluminescence detection.

All lanes:

Western blot - Anti-Granzyme B antibody [BLR022E] (ab243879) at 1/1000 dilution

Lane 1:

Myc-tagged Granzyme H overexpressing cell lysate at 15 µg

Lane 2:

Myc-tagged Granzyme B overexpressing cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 27.7 kDa

Observed band size: 38 kDa

false

Exposure time: 3s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

BLR022E

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, Flow Cyt (Intra), WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human GZMB.

P10144

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

产品详情

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

style
left-column

性能和储存信息

形式
Liquid
纯化说明
The monoclonal antibody was purified from cell culture supernatant.
存储溶液
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
See full target information GZMB
style
left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed41011180

2025

Granzyme B PET Imaging Enables Early Assessment of Immunotherapy Response in a Humanized Melanoma Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Priska Summer,Naomi Gallon,Niklas Bulmer,Umar Mahmood,Pedram Heidari

Frontiers in cell and developmental biology 12:1510264 PubMed39830210

2025

Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyao Chen,Shumin Cheng,Liang Gu,Zhangsen Huang,Changhua Zhang,Chunhui Sun,Songyao Chen

Journal of inflammation research 17:4505-4523 PubMed39006494

2024

Single-Cell Analysis Identifies Distinct Populations of Cytotoxic CD4 T Cells Linked to the Therapeutic Efficacy of Immune Checkpoint Inhibitors in Metastatic Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Yang,Jianwei Wu,Longlong Fan,Binghua Chen,Shiqiang Zhang,Wenzhong Zheng

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 65:1137-1143 PubMed38754959

2024

Granzyme B PET Imaging for Assessment of Disease Activity in Inflammatory Bowel Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pedram Heidari,Arvin Haj-Mirzaian,Suma Prabhu,Bahar Ataeinia,Shadi A Esfahani,Umar Mahmood

JCI insight 7: PubMed35290245

2022

Single-cell transcriptomics reveals distinct effector profiles of infiltrating T cells in lupus skin and kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett S Dunlap,Allison C Billi,Xianying Xing,Feiyang Ma,Mitra P Maz,Lam C Tsoi,Rachael Wasikowski,Jeffrey B Hodgin,Johann E Gudjonsson,J Michelle Kahlenberg,Deepak A Rao

Clinical cancer research : an official journal of the American Association for Cancer Research 27:5353-5364 PubMed34253581

2021

Non-invasive Detection of Immunotherapy-Induced Adverse Events.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina A Ferreira,Pedram Heidari,Bahar Ataeinia,Nicoleta Sinevici,Meghan E Sise,Robert B Colvin,Eric Wehrenberg-Klee,Umar Mahmood
View all publications
style
left-column
style
left-column
style
right-column

替代产品

Primary Antibodies

AB103159

Anti-Granzyme B antibody [CLB-GB11]

Flow Cytometry (Intracellular) - Anti-Granzyme B antibody [CLB-GB11] (AB103159)

  • 0
  • 0
Primary Antibodies

AB225185

Alexa Fluor® 647 Anti-Granzyme B antibody [EPR8260]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Granzyme B antibody [EPR8260] (AB225185)

  • 0
  • 0
Primary Antibodies

AB4059

Anti-Granzyme B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB4059)

  • 4
  • 22
Primary Antibodies

AB134933

Anti-Granzyme B antibody [EPR8260]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (AB134933)

  • 5
  • 2
Primary Antibodies

AB219803

Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (AB219803)

  • 4
  • 4
Primary Antibodies

AB214443

Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (AB214443)

  • 0
  • 0
Primary Antibodies

AB53097

Anti-Granzyme B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (AB53097)

  • 4
  • 5
Primary Antibodies

AB237847

Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [GZMB/2403] - BSA and Azide free (AB237847)

  • 0
  • 0
Primary Antibodies

AB208586

Anti-Granzyme B antibody [EPR20129-217]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR20129-217] (AB208586)

  • 0
  • 0

搭配使用产品

Assay Kits

AB235635

Human Granzyme B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Granzyme B ELISA Kit (AB235635)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19
Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587)

  • 5
  • 9
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com