Anti-Granzyme B抗体(ab53097)
Key features and details
- Rabbit polyclonal to Granzyme B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Granzyme B抗体
参阅全部 Granzyme B 一抗 -
描述
兔多克隆抗体to Granzyme B -
宿主
Rabbit -
特异性
Detects endogenous levels of total Granzyme B protein. Due to sequence homology this antibody is predicted to cross-react with granzyme H. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide (Human)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53097于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
|
IHC-P | (3) |
Use a concentration of 50 - 100 µg/ml.
|
说明 |
---|
WB
1/300 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
Use a concentration of 50 - 100 µg/ml. |
靶标
-
功能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 3002 Human
- Entrez Gene: 14939 Mouse
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P04187 Mouse
- Unigene: 1051 Human
-
别名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
图片
-
ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.
-
All lanes : Anti-Granzyme B antibody (ab53097) at 1/300 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing peptide
Predicted band size: 28 kDa
Observed band size: 28 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab53097 被引用在 9 文献中.
- Aslam MS et al. Therapeutical Significance of Serpina3n Subsequent Cerebral Ischemia via Cytotoxic Granzyme B Inactivation. Biomed Res Int 2022:1557010 (2022). PubMed: 35677097
- Zheng Y et al. PD-L1+CD8+ T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance. iScience 25:103785 (2022). PubMed: 35146396
- Jhuo CF et al. Attenuation of Tumor Development in Mammary Carcinoma Rats by Theacrine, an Antagonist of Adenosine 2A Receptor. Molecules 26:N/A (2021). PubMed: 34946538
- Sun Z et al. Norepinephrine inhibits the cytotoxicity of NK92-MI cells via the ß2-adrenoceptor/cAMP/PKA/p-CREB signaling pathway. Mol Med Rep 17:8530-8535 (2018). PubMed: 29658580
- Tahta A et al. Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turk Neurosurg 28:571-581 (2018). PubMed: 30192361
- Sun Y et al. Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice. Exp Ther Med 12:3773-3777 (2016). PubMed: 28105109
- Mohamedali KA et al. The functionalized human serine protease granzyme B/VEGF121 targets tumor vasculature and ablates tumor growth. Mol Cancer Ther 12:2055-66 (2013). ICC/IF . PubMed: 23858102
- Chaitanya GV et al. Role of cytotoxic protease granzyme-b in neuronal degeneration during human stroke. Brain Pathol 21:16-30 (2011). PubMed: 20825413
- Chaitanya GV et al. Granzyme-b is involved in mediating post-ischemic neuronal death during focal cerebral ischemia in rat model. Neuroscience 165:1203-16 (2010). WB ; Rat . PubMed: 19895873