Anti-Granzyme B抗体(ab4059)
Key features and details
- Rabbit polyclonal to Granzyme B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Granzyme B抗体
参阅全部 Granzyme B 一抗 -
描述
兔多克隆抗体to Granzyme B -
宿主
Rabbit -
特异性
The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig, Common marmoset
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (20) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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数据库链接
- Entrez Gene: 3002 Human
- Entrez Gene: 14939 Mouse
- Entrez Gene: 100233184 Pig
- Entrez Gene: 171528 Rat
- Omim: 123910 Human
- SwissProt: P10144 Human
- SwissProt: P04187 Mouse
- SwissProt: P18291 Rat
see all -
别名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)Huszthy et al PLoS One. 2015 Aug 20;10(8):e0136089. doi: 10.1371/journal.pone.0136089. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.
Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (116)
ab4059 被引用在 116 文献中.
- Lu W et al. Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer. EMBO Mol Med 13:e12798 (2021). PubMed: 33283987
- J Gunderson A et al. Germinal center reactions in tertiary lymphoid structures associate with neoantigen burden, humoral immunity and long-term survivorship in pancreatic cancer. Oncoimmunology 10:1900635 (2021). PubMed: 33796412
- Li H et al. The Beneficial Role of Sunitinib in Tumor Immune Surveillance by Regulating Tumor PD-L1. Adv Sci (Weinh) 8:2001596 (2021). PubMed: 33510997
- Liu X et al. Development of Facile and Versatile Platinum Drug Delivering Silicasome Nanocarriers for Efficient Pancreatic Cancer Chemo-Immunotherapy. Small 17:e2005993 (2021). PubMed: 33682329
- Liu X et al. Combination Chemo-Immunotherapy for Pancreatic Cancer Using the Immunogenic Effects of an Irinotecan Silicasome Nanocarrier Plus Anti-PD-1. Adv Sci (Weinh) 8:2002147 (2021). PubMed: 33747719