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Cell Biology Apoptosis Extracellular Signals Granzymes

Anti-Granzyme B抗体(ab4059)

  • Datasheet
  • SDS
Reviews (21)Q&A (22)References (116)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)

Key features and details

  • Rabbit polyclonal to Granzyme B
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Granzyme B antibody [EPR20129-217] (ab208586)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Granzyme B抗体
    参阅全部 Granzyme B 一抗
  • 描述

    兔多克隆抗体to Granzyme B
  • 宿主

    Rabbit
  • 特异性

    The immunogen used for this product shares 94% homology with Granzyme H. Cross-reactivity with this protein has not been confirmed experimentally.

  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Pig, Common marmoset
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Granzymes
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Innate Immunity
    • NK Cells
    • Neuroscience
    • Processes

相关产品

  • Assay kits

    • Granzyme B Activity Assay Kit (Fluorometric) (ab157403)
    • Granzyme B Inhibitor Screening Assay Kit (ab157405)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant mouse Granzyme B protein (ab50114)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4059于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (20)
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 序列相似性

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位

    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Target information above from: UniProt accession P10144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3002 Human
    • Entrez Gene: 14939 Mouse
    • Entrez Gene: 100233184 Pig
    • Entrez Gene: 171528 Rat
    • Omim: 123910 Human
    • SwissProt: P10144 Human
    • SwissProt: P04187 Mouse
    • SwissProt: P18291 Rat
    • Unigene: 1051 Human
    • Unigene: 132115 Rat
    • Unigene: 145789 Rat
    • Unigene: 21395 Rat
    see all
  • 别名

    • C11 antibody
    • Cathepsin G like 1 antibody
    • Cathepsin G-like 1 antibody
    • CCPI antibody
    • CGL 1 antibody
    • CGL1 antibody
    • CSP B antibody
    • CSPB antibody
    • CTLA 1 antibody
    • CTLA-1 antibody
    • CTLA1 antibody
    • CTSGL1 antibody
    • Cytotoxic serine protease B antibody
    • Cytotoxic T lymphocyte associated serine esterase 1 antibody
    • Cytotoxic T lymphocyte proteinase 2 antibody
    • Cytotoxic T-lymphocyte proteinase 2 antibody
    • Fragmentin 2 antibody
    • Fragmentin-2 antibody
    • GRAB_HUMAN antibody
    • Granzyme 2 antibody
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
    • Granzyme B antibody
    • Granzyme-2 antibody
    • GranzymeB antibody
    • GRB antibody
    • Gzmb antibody
    • Hlp antibody
    • Human lymphocyte protein antibody
    • Lymphocyte protease antibody
    • Protease, serine, B antibody
    • SECT antibody
    • T cell serine protease 1 3E antibody
    • T cell serine protease 1-3E antibody
    • T-cell serine protease 1-3E antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)Huszthy et al PLoS One. 2015 Aug 20;10(8):e0136089. doi: 10.1371/journal.pone.0136089. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Cytotoxic effector cells and regulatory T cells in human glioblastoma xenograft growing in immunocompetent rats.

    Granzyme B-positive cytotoxic cells (ab4059 at a 1/75 dilution) in a GBM xenograft with rejection. Asterisks mark tissue lyzed by effector cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody (ab4059)

    Human tonsil tissue stained with anti-Granzyme B antibody (ab4059).

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (116)

发表研究结果有使用 ab4059?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4059 被引用在 116 文献中.

  • Lu W  et al. Reprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer. EMBO Mol Med 13:e12798 (2021). PubMed: 33283987
  • J Gunderson A  et al. Germinal center reactions in tertiary lymphoid structures associate with neoantigen burden, humoral immunity and long-term survivorship in pancreatic cancer. Oncoimmunology 10:1900635 (2021). PubMed: 33796412
  • Li H  et al. The Beneficial Role of Sunitinib in Tumor Immune Surveillance by Regulating Tumor PD-L1. Adv Sci (Weinh) 8:2001596 (2021). PubMed: 33510997
  • Liu X  et al. Development of Facile and Versatile Platinum Drug Delivering Silicasome Nanocarriers for Efficient Pancreatic Cancer Chemo-Immunotherapy. Small 17:e2005993 (2021). PubMed: 33682329
  • Liu X  et al. Combination Chemo-Immunotherapy for Pancreatic Cancer Using the Immunogenic Effects of an Irinotecan Silicasome Nanocarrier Plus Anti-PD-1. Adv Sci (Weinh) 8:2002147 (2021). PubMed: 33747719
View all Publications for this product

客户评价及客户问答

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1-10 of 43 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Mar 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 19 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Specification
Spleen
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
10% Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

John Gillenwater

Verified customer

提交于 Apr 01 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsilla palatina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Tonsilla palatina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

提交于 Jan 25 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Victor Bernal

Verified customer

提交于 Aug 16 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Regressing histiocytoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Heat-induced with Bond TM Epitope Retrieval 1 (Leica AR9961) Solution for 20 minutes at 95 C
Permeabilization
Yes - Yes (ProClinTM 950)
Specification
Regressing histiocytoma
Blocking step
PowerVision IHC/ISH Super Blocking (Leica PV6122) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
10% Neutral Buffered Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 07 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Mar 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Mammary gland tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
Mammary gland tumor
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 18 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Adipose tissue
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Feb 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Granzyme B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Feb 17 2019

1-10 of 43 Abreviews or Q&A

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