Anti-Grainyhead-like蛋白1 homolog抗体(ab111582)
Key features and details
- Rabbit polyclonal to Grainyhead-like protein 1 homolog
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Grainyhead-like蛋白1 homolog抗体 -
描述
兔多克隆抗体to Grainyhead-like蛋白1 homolog -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-208 of Human Grainyhead-like protein 1 homolog.
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阳性对照
- NT2D1, U-87 MG and MCF7 cells and whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab111582 is purified by antigen affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111582于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Transcription factor. Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed. May play a role in development. May be involved in epidermal differentiation (By similarity). Binds and transactivates the EN1 promoter. -
组织特异性
Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Highly expressed in placental JEG-3 cells and at very low levels in non-steroidogenic cells. No expression detected in adrenal NCI-H295A cells. -
序列相似性
Belongs to the grh/CP2 family. Grainyhead subfamily. -
发展阶段
Expressed in fetal liver and brain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29841 Human
- Omim: 609786 Human
- SwissProt: Q9NZI5 Human
- Unigene: 418493 Human
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别名
- Grainyhead-like protein 1 homolog antibody
- grhl1 antibody
- GRHL1_HUMAN antibody
see all
图片
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Western blot - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)Anti-Grainyhead-like protein 1 homolog antibody (ab111582) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-Grainyhead-like protein 1 homolog antibody (ab111582)ab111582 at 1/500 dilution staining Grainyhead-like protein 1 homolog in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab111582 尚未被引用在任何文献中。