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重组RabMAb

重组Anti-GPX8抗体[EPR28823-80] - BSA and Azide free (ab317745)

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Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
  • Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28823-80] to GPX8 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-GPX8抗体[EPR28823-80] - BSA and Azide free
    参阅全部 GPX8 一抗

  • 描述

    兔单克隆抗体[EPR28823-80] to GPX8 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Unsuitable for mouse ICC

  • 经测试应用

    适用于: ICC/IF, IP, WBmore details
    不适用于: Flow Cyt or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, U-2 OS, 293T, NIH/3T3, C2C12, Human uterus, Rat uterus, 293T cells transfected with a human GPX8 expression vector containing a His-tag, whole lysates. ICC/IF: HeLa cells. IP: HeLa cells.

  • 常规说明

    ab317745 is the carrier-free version of ab317744

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317745于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
说明
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
应用说明
Is unsuitable for Flow Cyt or IHC-P.

靶标

图片

  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    All lanes : Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
    Lane 6 : 293T (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HepG2 (PMID: 32817494), Jurkat

    Low expression: MCF7(PMID:32817494)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    All lanes : Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast) whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Neuro-2a

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    All lanes : Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1 : Human uterus tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Rat uterus tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Western blot - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    All lanes : Anti-GPX8 antibody [EPR28823-80] (ab317744) at 1/1000 dilution

    Lane 1 : 293T cells transfected with an empty vector containing a His-tag, whole cell lysate
    Lane 2 : 293T cells transfected with a human GPX7 expression vector containing a His-tag, whole cell lysate
    Lane 3 : 293T cells transfected with a human GPX8 expression vector containing a His-tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 24 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?


    Exposure time: 6 seconds


    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human GPX7.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade  (ab213204) staining at 1/5000 dilution.

  • Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Immunoprecipitation - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)

    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    GPX8 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317744 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317744 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab317744 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317744 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Immunocytochemistry/ Immunofluorescence - Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)

    This data was developed using ab317744, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GPX8 with ab317744 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing positive staining in Hela cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
    Negative control: HepG2 (PMID: 32817494).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)
    Anti-GPX8 antibody [EPR28823-80] - BSA and Azide free (ab317745)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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