重组Anti-GPR56抗体[EPR23908-115] (ab274419)
![Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)](https://www.abcam.cn/ps/products/274/ab274419/Images/ab274419-397795-anti-gpr56-antibody-epr23908115-western-blot-lysate1.jpg "Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23908-115] to GPR56
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GPR56抗体[EPR23908-115]
参阅全部 GPR56 一抗 -
描述
兔单克隆抗体[EPR23908-115] to GPR56 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human brain, hippocampus and glioma tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23908-115 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 78 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 78 kDa). |
靶标
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功能
Could be involved in cell-cell interactions. -
组织特异性
Widely distributed with highest levels found in thyroid gland, brain and heart. Expressed in a great number of tumor cells. -
疾病相关
Polymicrogyria, bilateral frontoparietal (BFPP) [MIM:606854]: A malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination, most severe in the frontoparietal regions. BFPP clinical manifestations include developmental and psychomotor delay, cerebellar and pyramidal signs, truncal ataxia, seizures, hyperreflexia. Polymicrogyria is a heterogeneous disorder, considered to be the result of postmigratory abnormal cortical organization. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain. -
翻译后修饰
The endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9289 Human
- Omim: 604110 Human
- SwissProt: Q9Y653 Human
- Unigene: 513633 Human
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别名
- 7 transmembrane protein with no EGF like N terminal domains 1 antibody
- BFPP antibody
- DKFZp781L1398 antibody
see all
图片
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Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution + Human glioma tissue lysate at 10 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 70 seconds.
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![Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)](https://www.abcam.cn/ps/products/274/ab274419/Images/ab274419-397794-anti-gpr56-antibody-epr23908115-western-blot-lysate.jpg)
Western blot - Anti-GPR56 antibody [EPR23908-115] (ab274419)All lanes : Anti-GPR56 antibody [EPR23908-115] (ab274419) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human lung tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 17932623).
Negative control: Human lung (PMID: 15674329)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
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![Anti-GPR56 antibody [EPR23908-115] (ab274419)](https://www.abcam.cn/ps/products/274/ab274419/Images/ab274419-4-benefits-of-recombinant-antibodies.png)
Anti-GPR56 antibody [EPR23908-115] (ab274419)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab274419 尚未被引用在任何文献中。