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AB272357

重组Anti-GPNMB抗体[EPR22150-10] - BSA and Azide free (Detector)

Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal GPNMB antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

Dchil, Hgfin, Nmb, Gpnmb, Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin

1 Images
Sandwich ELISA - Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector) (AB272357)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-GPNMB antibody [EPR22150-10] - BSA and Azide free (Detector) (AB272357)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270892).

不同偶联物与剂型 (1)

  • Biotin

    Biotin Anti-GPNMB antibody [EPR22150-10] (Detector)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22150-10

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB270892

Mouse GPNMB ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse GPNMB ELISA Kit (AB270892)

  • 0
  • 0
Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

  • 0
  • 0
Sample Prep & Detection Kits

AB270228

Europium-Streptavidin

Functional Studies - Europium-Streptavidin (AB270228)

  • 5
  • 1
Primary Antibodies

AB281203

Anti-Alpha-synuclein antibody [EPR23675-102] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Alpha-synuclein antibody [EPR23675-102] - BSA and Azide free (Capture) (AB281203)

  • 0
  • 0
Primary Antibodies

AB285119

Anti-IP10 antibody [EPR24674-2] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IP10 antibody [EPR24674-2] - BSA and Azide free (Detector) (AB285119)

  • 0
  • 0
Primary Antibodies

AB259622

Anti-CHIT1 antibody [EPR22315-210] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CHIT1 antibody [EPR22315-210] - BSA and Azide free (Detector) (AB259622)

  • 0
  • 0
Primary Antibodies

AB259752

Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (Detector) (AB259752)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab272357 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse GPNMB. The recommended pair for sandwich ELISA is:
Capture: ab272356, Mouse GPNMB Capture Antibody (unconjugated)
Detector: ab272357, Mouse GPNMB Detector Antibody (unconjugated)
The reference range value is 0.78125 - 50 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GPNMB also known as osteoactivin is a type I transmembrane glycoprotein with a molecular mass of approximately 115 kDa. This protein is highly expressed in various tissues including the bone skin and brain. It also shows notable presence in certain immune cells such as dendritic cells and macrophages. GPNMB localizes mainly on the cell membrane and within lysosomal compartments indicating its involvement in cellular and signaling processes.
Biological function summary

GPNMB plays versatile roles in promoting cell growth differentiation and repair. It is involved in processes like tissue regeneration possibly due to its role in cell adhesion and migration. The protein also partakes in osteoclast differentiation and bone resorption therefore linking to skeletal homeostasis. GPNMB forms part of a broader protein complex at the cellular junctions interacting with integrins to mediate its functions.

Pathways

GPNMB features prominently in osteoclastogenesis and melanoma metastasis pathways. It cooperates with RANKL an important protein in bone metabolism by influencing osteoclast differentiation and activation. In the context of melanoma GPNMB's action in pathways may enhance tumor proliferation and invasion possibly interacting with signaling molecules like AKT or ERK although the specifics remain actively researched.

GPNMB has significant links to both osteoarthritis and certain types of cancer particularly breast cancer and melanoma. In osteoarthritis GPNMB expression often increases reflecting involvement in cartilage degradation processes. Its overexpression correlates with tumor progression in melanoma potentially driven by its interaction with integrin β1 which assists in cellular adhesion and migration within the tumor microenvironment.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Could be a melanogenic enzyme.
See full target information Gpnmb
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