重组Anti-GPD2抗体[EPR14260] (ab182144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14260] to GPD2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-GPD2抗体[EPR14260]
参阅全部 GPD2 一抗 -
描述
兔单克隆抗体[EPR14260] to GPD2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- NIH:OVCAR-3 and MCF-7 cell lysate. Human pancreas tissue. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14260 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182144于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/140.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/140. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 81 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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通路
Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. -
序列相似性
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Contains 2 EF-hand domains. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 2820 Human
- Omim: 138430 Human
- SwissProt: P43304 Human
- Unigene: 512382 Human
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别名
- GDH2 antibody
- Glycerol 3 phosphate dehydrogenase 2 (mitochondrial) antibody
- Glycerol 3 phosphate dehydrogenase 2 antibody
see all
图片
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All lanes : Anti-GPD2 antibody [EPR14260] (ab182144) at 1/2000 dilution
Lane 1 : NIH:OVCAR-3 cell lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 81 kDa -
Immunohistochemical analysis of paraffin embedded Human pancreas tissue sections labeling GPD2 using ab182144 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HeLa cells labeling GPD2 using ab182144 at a 1/140 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab182144 被引用在 1 文献中.
- Wu ST et al. Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI. Front Pharmacol 11:379 (2020). PubMed: 32292350