Anti-GPCR RDC1/CXCR-7抗体(ab72100)
Key features and details
- Rabbit polyclonal to GPCR RDC1/CXCR-7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GPCR RDC1/CXCR-7抗体
参阅全部 GPCR RDC1/CXCR-7 一抗 -
描述
兔多克隆抗体to GPCR RDC1/CXCR-7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 106-117.
Sequence:QHNQWPMGELTC
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72100于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. -
组织特异性
Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 57007 Human
- Entrez Gene: 12778 Mouse
- Omim: 610376 Human
- SwissProt: P25106 Human
- SwissProt: P56485 Mouse
- Unigene: 471751 Human
- Unigene: 6522 Mouse
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别名
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml
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Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with immunizing peptide
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 29 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/ml
Lane 1 : Human spleen
Lane 2 : Mouse spleen
Lane 3 : Human tonsil
Lane 4 : Human lymph node
Secondary
All lanes : anti-rabbit HRP
Predicted band size: 42 kDaBlocking buffer: 5% non-fat milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab72100 被引用在 39 文献中.
- Chen WS et al. Ginkgolide A improves the pleiotropic function and reinforces the neuroprotective effects by mesenchymal stem cell-derived exosomes in 6-OHDA-induced cell model of Parkinson's disease. Aging (Albany NY) 15:1358-1370 (2023). PubMed: 36863713
- Wang D et al. Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts. Cell Death Dis 13:380 (2022). PubMed: 35443745
- Si M et al. CXCL12/CXCR7/β-arrestin1 biased signal promotes epithelial-to-mesenchymal transition of colorectal cancer by repressing miRNAs through YAP1 nuclear translocation. Cell Biosci 12:171 (2022). PubMed: 36210463
- Babst N et al. CXCR4, CXCR5 and CD44 May Be Involved in Homing of Lymphoma Cells into the Eye in a Patient Derived Xenograft Homing Mouse Model for Primary Vitreoretinal Lymphoma. Int J Mol Sci 23:N/A (2022). PubMed: 36233057
- Daryabari SS et al. Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models. J Assist Reprod Genet 39:1237-1247 (2022). PubMed: 35513746