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AB117836

Anti-GPCR RDC1/CXCR-7抗体

Anti-GPCR RDC1/CXCR-7 antibody

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(7 Publications)

Rabbit Polyclonal GPCR RDC1/CXCR-7 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ackr3 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

查看别名

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)

Immunofluorescence analysis of CXCR7 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCR7 Rabbit Polyclonal Antibody (ab117836) at 1/1000 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin (1/300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)

Immunocytochemistry analysis of HEK293 cells using 1/5000 ab117836.

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)
  • WB

Supplier Data

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)

The blots were probed with Anti-CXCR7 Rabbit Polyclonal Antibody (ab117836, 1/4000-1/6000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 μg/mL, 1/2500 dilution).

Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab117836)

Lane 1:

Mouse brain whole cell lysate at 20 µg

Lane 2:

Rat brain whole cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)
  • WB

Unknown

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (AB117836)

All lanes:

Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab117836) at 1/5000 dilution

All lanes:

Mouse brain lysate

Predicted band size: 41 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Mouse Ackr3 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P56485

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯度
Whole antiserum
存储溶液
Preservative: 0.02% Sodium azide Constituents: 99% Whole serum
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GPCR RDC1 also known as CXCR-7 is a G protein-coupled receptor that binds to chemokines such as CXCL12 and CXCL11. The receptor has a predicted molecular mass of approximately 40 kDa. It expresses widely in various tissues with high levels found in the heart liver kidney and brain. This receptor plays a role in the trafficking and localization of different cells often working alongside or complementing other chemokine receptors.
Biological function summary

GPCR RDC1/CXCR-7 takes part in complex cell signaling related to cell growth survival and migration. It is not usually part of a classic GPCR signaling pathway which sets it apart from other receptors in its family. Instead it can modulate pathways by forming heterodimers with other receptors such as CXCR4 affecting both ligand availability and receptor sensitivity. CXCR-7 can also function as a decoy receptor binding ligands without initiating a typical signaling cascade.

Pathways

The participation of GPCR RDC1/CXCR-7 is noted in chemokine-mediated pathways. Of particular importance is the SDF-1/CXCL12 signaling pathway which it shares with CXCR4. This pathway plays an important role in the regulation of stem cell migration and homing. CXCR-7 modulates the bioavailability of CXCL12 and influences how CXCR4 triggers downstream effects affecting cellular response to the external environment.

GPCR RDC1/CXCR-7 holds significance in cancer and cardiovascular diseases. In cancer overexpression of CXCR-7 associates with tumor growth and metastasis often in conjunction with the action of CXCR4. In cardiovascular conditions altered expression and functional shifts in CXCR-7 can lead to abnormal vascularization contributing to disease progression. Its role in these diseases makes CXCR-7 a valuable target for therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed : 16107333, PubMed : 19255243, PubMed : 19380869, PubMed : 20161793, PubMed : 22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed : 16940167, PubMed : 18653785, PubMed : 20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed : 16940167, PubMed : 18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed : 16940167, PubMed : 20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed : 17804806, PubMed : 18653785, PubMed : 19641136, PubMed : 20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed : 18653785). Required for heart valve development (PubMed : 17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed : 31211835).. (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.
See full target information ACKR3
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70352 PubMed39855896

2025

Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfeng Lu,Yuting Zhang,Cuilian Sun,Yuhang Na,Haotian Sun,Jianhua Ma,Xueqin Wang,Xiaomin Cang

Journal of experimental & clinical cancer research : CR 42:297 PubMed37950281

2023

Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshi Wang,Dehai Wang,Yanan Sun,Ting Zhuang,Xin Li,Huijie Yang,Yifeng Zang,Ziping Liu,Penghe Yang,Chenmiao Zhang,Jiayao Cui,Mingxi Fu,Shuqing Zhang,Peng Su,Zhongbo Li,Jian Zhu,Yinlu Ding

Nature communications 13:1481 PubMed35304461

2022

S100A9-CXCL12 activation in BRCA1-mutant breast cancer promotes an immunosuppressive microenvironment associated with resistance to immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjie Li,Xiaodong Shu,Jun Xu,Sek Man Su,Un In Chan,Lihua Mo,Jianlin Liu,Xin Zhang,Ragini Adhav,Qiang Chen,Yuqing Wang,Tingting An,Xu Zhang,Xueying Lyu,Xiaoling Li,Josh Haipeng Lei,Kai Miao,Heng Sun,Fuqiang Xing,Aiping Zhang,Chuxia Deng,Xiaoling Xu

Cells 9: PubMed32560483

2020

Beneficial Effect of IL-4 and SDF-1 on Myogenic Potential of Mouse and Human Adipose Tissue-Derived Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Archacka,Joanna Bem,Edyta Brzoska,Areta M Czerwinska,Iwona Grabowska,Paulina Kasprzycka,Dzesika Hoinkis,Katarzyna Siennicka,Zygmunt Pojda,Patrycja Bernas,Robert Binkowski,Kinga Jastrzebska,Aleksandra Kupiec,Malgorzata Malesza,Emilia Michalczewska,Marta Soszynska,Katarzyna Ilach,Wladyslawa Streminska,Maria A Ciemerych

International journal of molecular sciences 21: PubMed32392778

2020

IL-4 and SDF-1 Increase Adipose Tissue-Derived Stromal Cell Ability to Improve Rat Skeletal Muscle Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Małgorzata Zimowska,Karolina Archacka,Edyta Brzoska,Joanna Bem,Areta M Czerwinska,Iwona Grabowska,Paulina Kasprzycka,Emilia Michalczewska,Igor Stepaniec,Marta Soszynska,Katarzyna Ilach,Wladyslawa Streminska,Maria A Ciemerych

Scientific reports 5:16813 PubMed26578388

2015

Crosstalk between SDF-1/CXCR4 and SDF-1/CXCR7 in cardiac stem cell migration.

Applications

WB

Species

Unspecified reactive species

Dong Chen,Yanli Xia,Ke Zuo,Ying Wang,Shiying Zhang,Dong Kuang,Yaqi Duan,Xia Zhao,Guoping Wang

Stem cell research & therapy 6:46 PubMed25890097

2015

Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration.

Applications

WB, ICC

Species

Unspecified reactive species, Unspecified reactive species

Edyta Brzoska,Kamil Kowalski,Agnieszka Markowska-Zagrajek,Magdalena Kowalewska,Rafał Archacki,Izabela Plaskota,Władysława Stremińska,Katarzyna Jańczyk-Ilach,Maria A Ciemerych
View all publications
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