Anti-GPCR GPR43抗体(ab124272)
Key features and details
- Rabbit polyclonal to GPCR GPR43
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR43抗体
参阅全部 GPCR GPR43 一抗 -
描述
兔多克隆抗体to GPCR GPR43 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Synthetic peptide conjugated to KLH, corresponding to the C terminal region of Human GPCR GPR43.
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阳性对照
- Human tonsil tissue; COS7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124272于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. -
组织特异性
Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2867 Human
- Omim: 603823 Human
- SwissProt: O15552 Human
- Unigene: 248056 Human
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别名
- Fatty acid receptor 2 antibody
- FFA2R antibody
- Ffar2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR43 antibody (ab124272)ab124272, at 5 µg/ml, staining GPCR GPR43 in formalin fixed, embedded Human tonsil tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR43 antibody (ab124272)ab124272, at a 1/100 dilution, staining GPCR GPR43 in COS7 cells by iImmunofluorescence.
The image on the right is treated with the synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124272 被引用在 3 文献中.
- Frei R et al. Allosteric targeting of the FFA2 receptor (GPR43) restores responsiveness of desensitized human neutrophils. J Leukoc Biol 109:741-751 (2021). PubMed: 32803826
- Matsuya-Ogawa M et al. Oncoprotective Effects of Short-Chain Fatty Acids on Uterine Cervical Neoplasia. Nutr Cancer 71:312-319 (2019). PubMed: 30836015
- Pan P et al. Loss of free fatty acid receptor 2 enhances colonic adenoma development and reduces the chemopreventive effects of black raspberries in ApcMin/+ mice. Carcinogenesis 38:86-93 (2017). PubMed: 27866157