AB174835

重组Anti-GPCR GPR18抗体[EPR12359]

Anti-GPCR GPR18 antibody [EPR12359]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal GPCR GPR18 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

查看别名

GPCRW, GPR18, N-arachidonyl glycine receptor, NAGly receptor, G-protein coupled receptor 18

1 Images

Supplier Data

Western blot - Anti-GPCR GPR18 antibody [EPR12359] (AB174835)

All lanes:

Western blot - Anti-GPCR GPR18 antibody [EPR12359] (ab174835) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

true

不同偶联物与剂型 (1)

Carrier free

Anti-GPCR GPR18 antibody [EPR12359] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12359

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GPR18 also known as N-arachidonyl glycine receptor is a G protein-coupled receptor (GPCR) found in various human tissues. It has an approximate mass of 40 kDa. This receptor is expressed most notably in immune cells including lymphocytes and macrophages as well as in the spleen and thymus. In addition to immune tissues GPR18 is also present in the heart brain and gastrointestinal system.

Biological function summary

This receptor plays a role in cellular signaling as part of the cardiovascular and immune system regulation. GPR18 binds specific ligands such as N-arachidonoyl glycine facilitating intracellular communication and response to environmental changes. It does not function alone but integrates into GPCR complexes acting in concert with other receptors to regulate physiological functions like inflammation and cell migration.

Pathways

The involvement of GPR18 in the endocannabinoid signaling and in the immune system makes it an essential player. It interfaces significantly with the endocannabinoid system influencing pathways like the calcium signaling pathway and possibly the MAPK signaling pathway. GPR18 interacts with proteins such as CB2 (cannabinoid receptor 2) to modulate immune responses inflammation and other critical cellular processes.

GPR18 is associated with conditions like inflammation and cardiovascular diseases. Changes in its expression or function can contribute to the development of these health issues. The receptor's interaction with proteins like CB2 becomes evident in these contexts where it may modulate disease progression and provide potential therapeutic targets for treating inflammation-related disorders or heart diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G protein-coupled receptor (GPCR) that plays a role in diverse physiological processes particularly within the immune and nervous systems (PubMed : 21732409, PubMed : 26195725). Becomes active when triggered by various endogenous ligands including endocannabinoid N-arachidonyl glycine (NAGly), delta-9-tetrahydrocannabinol or resolvin D2/RvD2 derived from the omega-3 fatty acid docosahexaenoic acid (DHA) (PubMed : 16844083, PubMed : 24762058, PubMed : 26195725, PubMed : 27572937). Upon RvD2 binding, facilitates the resolution of inflammation, aiding in tissue repair and homeostasis. Mechanistically, RvD2 ligation initiates Galphas protein coupling, activation of cAMP-PKA signaling pathway and phosphorylation of STAT3, leading to RvD2-stimulated macrophage phagocytosis (PubMed : 27994074). Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed : 27572937). Activation by N-arachidonoyl glycine (NAGly) can also induce apoptosis in macrophages (By similarity). Plays a role in homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs) in small intestine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity). Also participates in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity).

See full target information GPR18

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1458684 PubMed39380984

2024

Activation of pro-resolving pathways mediate the therapeutic effects of thymosin beta-4 during -induced keratitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Wang,Loveleen Banga,Abdul Shukkur Ebrahim,Thomas W Carion,Gabriel Sosne,Elizabeth A Berger

Drug design, development and therapy 15:4687-4699 PubMed34815664

2021

Activation of GPR18 by Resolvin D2 Relieves Pain and Improves Bladder Function in Cyclophosphamide-Induced Cystitis Through Inhibiting TRPV1.

Applications

Unspecified application

Species

Unspecified reactive species

Qudong Lu,Yang Yang,Hengshuai Zhang,Cheng Chen,Jiang Zhao,Zhenxing Yang,Yi Fan,Longkun Li,Huan Feng,Jingzhen Zhu,Shanhong Yi

International journal of molecular sciences 21: PubMed32075117

2020

Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Patryk Remiszewski,Iwona Jarocka-Karpowicz,Michał Biernacki,Anna Jastrząb,Eberhard Schlicker,Marek Toczek,Ewa Harasim-Symbor,Anna Pędzińska-Betiuk,Barbara Malinowska

Circulation 134:666-680 PubMed27507404

2016

Resolvin D2 Enhances Postischemic Revascularization While Resolving Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Zhang,Brian E Sansbury,Jason Hellmann,James F Baker,Luping Guo,Caitlin M Parmer,Joshua C Prenner,Daniel J Conklin,Aruni Bhatnagar,Mark A Creager,Matthew Spite

View all publications

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重组Anti-GPCR GPR18抗体[EPR12359]