Anti-GPCR GPR125抗体(ab51705)
Key features and details
- Rabbit polyclonal to GPCR GPR125
- Suitable for: ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR125抗体 -
描述
兔多克隆抗体to GPCR GPR125 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, ELISAmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide:
TKRGNNTRTVDIVVLESSAQYCPPERV
conjugated to KLH, corresponding to internal sequence amino acids 328-354 of Human GPCR GPR125 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/200.
|
|
| ELISA |
Use a concentration of 0.001 - 0.1 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
1/200. |
|
ELISA
Use a concentration of 0.001 - 0.1 µg/ml. |
靶标
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功能
Orphan receptor that may have a role in planar cell polarity pathway. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 1 GPS domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 5 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 166647 Human
- Entrez Gene: 70693 Mouse
- Omim: 612303 Human
- SwissProt: Q8IWK6 Human
- SwissProt: Q7TT36 Mouse
- Unigene: 684429 Human
- Unigene: 99195 Human
- Unigene: 272974 Mouse
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别名
- Adgra3 antibody
- Adhesion G protein-coupled receptor A3 antibody
- AGRA3_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR125 antibody (ab51705)ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ELISA - Anti-GPCR GPR125 antibody (ab51705)ELISA using ab51705 at varying antibody concentrations and antigen concentration at 1000 ng/mL.
数据表及文件
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Datasheet download
文献 (15)
ab51705 被引用在 15 文献中.
- Guihurt Santiago J et al. Adhesion G protein-coupled receptor, ELTD1, is a potential therapeutic target for retinoblastoma migration and invasion. BMC Cancer 21:53 (2021). PubMed: 33430814
- Chen W et al. Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2. Mol Ther Nucleic Acids 20:788-800 (2020). PubMed: 32438314
- Fu H et al. PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways. Mol Ther Nucleic Acids 12:769-786 (2018). PubMed: 30141410
- Zhao L et al. VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2. Cell Cycle 17:225-239 (2018). PubMed: 29169284
- Wu Y et al. Elevated G-Protein Receptor 125 (GPR125) Expression Predicts Good Outcomes in Colorectal Cancer and Inhibits Wnt/ß-Catenin Signaling Pathway. Med Sci Monit 24:6608-6616 (2018). PubMed: 30231258
