Anti-GPCR GPR116抗体(ab111169)
Key features and details
- Rabbit polyclonal to GPCR GPR116
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR116抗体
参阅全部 GPCR GPR116 一抗 -
描述
兔多克隆抗体to GPCR GPR116 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide: V CYKHNFNA SS VSWCSKT VDV CCHFTN AANN SVWSP SMKLN LVPG ENITCQ, corresponding to amino acids 620-670 of Human GPCR GPR116
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阳性对照
- Human breast carcinoma tissue; A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab111169 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111169于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
May have a role in the regulation of acid-base balance. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 3 Ig-like (immunoglobulin-like) domains.
Contains 1 SEA domain. -
翻译后修饰
Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 221395 Human
- SwissProt: Q8IZF2 Human
- Unigene: 362806 Human
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别名
- DKFZp564O1923 antibody
- FLJ90640 antibody
- G protein coupled receptor 116 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR116 antibody (ab111169)ab111169 at 1/50 dilution staining GPCR GPR116 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR116 antibody (ab111169)ab111169 at 1/100 dilution staining GPCR GPR116 in A549 cells by Immunofluorescence. The image on the right is treated with synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab111169 被引用在 3 文献中.
- Georgiadi A et al. Orphan GPR116 mediates the insulin sensitizing effects of the hepatokine FNDC4 in adipose tissue. Nat Commun 12:2999 (2021). PubMed: 34016966
- Bouvier C et al. Recurrent novel THBS1-ADGRF5 gene fusion in a new tumor subtype "Acral FibroChondroMyxoid Tumors". Mod Pathol 33:1360-1368 (2020). PubMed: 32047233
- Tang X et al. GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the Gaq-p63RhoGEF-Rho GTPase pathway. Cancer Res 73:6206-18 (2013). IHC ; Human . PubMed: 24008316