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重组RabMAb

重组Anti-GPA33抗体[EPR4240] (ab108938)

Reviews (2)Q&A (1)References (6)
Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
  • Anti-GPA33 antibody [EPR4240] (ab108938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4240] to GPA33
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-GPA33抗体[EPR4240]
    参阅全部 GPA33 一抗

  • 描述

    兔单克隆抗体[EPR4240] to GPA33

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human small intestine, colon and fetal colon tissue lysates; HT-29 and MOLT-4 cell lysates; IHC-P: Human colon tissue. Human colon cancer tissue. Human ovarian cancer tissue; IP: HT-29 cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4240

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108938于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000. Predicted molecular weight: 36 kDa.

For unpurified use at 1/1000 - 1/10000.
IP
1/20.

For unpurified use at 1/10 - 1/100.
IHC-P (1)
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.
说明
WB
1/1000. Predicted molecular weight: 36 kDa.

For unpurified use at 1/1000 - 1/10000.
IP
1/20.

For unpurified use at 1/10 - 1/100.
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

靶标

  • 功能

    May play a role in cell-cell recognition and signaling.

  • 组织特异性

    Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻译后修饰

    N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
    Palmitoylated.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    图片

    • Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
      Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
      All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (Purified)

      Lane 1 : Human colon lysates
      Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking/Diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling GPA33 with Purified ab108938 at 1:400 dilution (0.25 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunoprecipitation - Anti-GPA33 antibody [EPR4240] (ab108938)

      Purified ab108938 at 1:20 dilution (0.5ug) immunoprecipitating GPA33 in HT-29 whole cell lysate.

      Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate (10µg)

      Lane 2 (+): ab108938 + HT-29 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108938 in HT-29 whole cell lysate.

      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.

      Blocking Buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

      Observed band size: 50 kDa

    • Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
      Western blot - Anti-GPA33 antibody [EPR4240] (ab108938)
      All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (unpurified)

      Lane 1 : HT-29 lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : Human fetal colon lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 at 1/250 dilution staining GPA33 in paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 showing negative staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 showing negative staining in Thyroid gland carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 showing positive staining in Ovarian carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 showing negative staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] (ab108938)

      Unpurified ab108938 showing negative staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-GPA33 antibody [EPR4240] (ab108938)
      Anti-GPA33 antibody [EPR4240] (ab108938)

    文献 (6)

    发表研究结果有使用 ab108938？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108938 被引用在 6 文献中.

    • Luk IY  et al. Epithelial de-differentiation triggered by co-ordinate epigenetic inactivation of the EHF and CDX1 transcription factors drives colorectal cancer progression. Cell Death Differ 29:2288-2302 (2022). PubMed: 35606410
    • Voigt RM  et al. Abnormal intestinal milieu in posttraumatic stress disorder is not impacted by treatment that improves symptoms. Am J Physiol Gastrointest Liver Physiol 323:G61-G70 (2022). PubMed: 35638693
    • Zhang Q  et al. Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets. Nat Cell Biol 23:1240-1254 (2021). PubMed: 34887515
    • Lopes N  et al. A panel of intestinal differentiation markers (CDX2, GPA33, and LI-cadherin) identifies gastric cancer patients with favourable prognosis. Gastric Cancer 23:811-823 (2020). PubMed: 32215766
    • Gotanda K  et al. Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. Sci Rep 6:32299 (2016). WB, IP . PubMed: 27571936
    • Jiang L  et al. EpCAM-dependent extracellular vesicles from intestinal epithelial cells maintain intestinal tract immune balance. Nat Commun 7:13045 (2016). PubMed: 27721471

    客户评价及客户问答

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GPA33 antibody [EPR4240]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat
    Permeabilization
    No
    Specification
    Colon tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Fabia Fricke

    Verified customer

    提交于 Dec 18 2017

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