AB74579

Anti-GORASP2/GRASP55抗体

Name: GORASP2/GRASP55抗体 (ab74579)| Abcam中文官网
Brand: Abcam Limited
SKU: AB74579
Price: 4606 CNY
Availability: InStock

Anti-GORASP2/GRASP55 antibody

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(2 Publications)

Rabbit Polyclonal GORASP2/GRASP55 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GORASP2.

查看别名

GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55

1 Images

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Western blot - Anti-GORASP2/GRASP55 antibody (AB74579)

All lanes:

Western blot - Anti-GORASP2/GRASP55 antibody (ab74579) at 1/500 dilution

Lane 1:

HeLa cell extracts at 10 µg

Lane 2:

HepG2 cell extracts at 10 µg

Lane 3:

Jurkat cell extracts at 10 µg

Lane 4:

LOVO cell extracts at 10 µg

Lane 5:

HeLa cell extracts at 10 µg with immunizing peptide

Predicted band size: 47 kDa

Observed band size: 47 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human GORASP2. The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab74579 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GORASP2 also known as GRASP55 is a golgi reassembly stacking protein with a mass approximately 55 kDa. It is highly expressed in the Golgi apparatus where it plays an important role in maintaining the structure and function of this organelle. GRASP55 facilitates the stacking of Golgi cisternae which is essential for Golgi apparatus organization. The protein acts alongside its isoform GRASP65 though they function somewhat independently in different Golgi regions.

Biological function summary

GRASP55 controls various cellular processes including the trafficking of proteins and lipids within the cell. It is part of the GRASP complex along with GRASP65 facilitating membrane tethering and fusion events. The protein's function is also linked to its ability to regulate Golgi's response to cellular stress or changes in environment impacting secretion and growth factor availability. In some conditions GRASP55 can influence processes such as autophagy underlining its diverse biological actions within the cell.

Pathways

GRASP55 integrates into significant cellular pathways like the secretory pathway and the autophagy pathway. It interacts with key proteins like golgins and Rab GTPases to assist in vesicle tethering and transport. GRASP55 also closely interacts with p62 a protein that plays a critical role in the selective autophagy pathway. This positions GRASP55 as a contributor to not only the routine processing of cellular materials but also cellular adaptation to stress through autophagy modulation.

GRASP55 shows relevance to cancer progression and neurodegenerative diseases. Aberrant expression or dysfunction of GRASP55 can contribute to cancerous transformations by altering cell adhesion and signaling pathways. Its role in autophagy connects it to diseases like Alzheimer's where defective protein processing is a hallmark. The interaction of GRASP55 with proteins such as p62 becomes particularly important as disrupted autophagy pathways are often implicated in neurodegenerative conditions highlighting GRASP55's significant role in both health and disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Key structural protein of the Golgi apparatus (PubMed : 33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed : 33301566). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed : 33301566). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed : 10487747, PubMed : 21515684, PubMed : 22523075). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (PubMed : 11101516). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed : 21884936, PubMed : 27062250, PubMed : 28067262).

See full target information GORASP2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105320 PubMed35748162

2022

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Applications

Unspecified application

Species

Unspecified reactive species

Hak Park,Soo Kyung Seo,Ju-Ri Sim,Su Jin Hwang,Ye Jin Kim,Dong Hoon Shin,Dong Geon Jang,Shin Hye Noh,Pil-Gu Park,Si Hwan Ko,Mi Hwa Shin,Jae Young Choi,Yukishige Ito,Chung-Min Kang,Jae Myun Lee,Min Goo Lee

Autophagy 14:1761-1778 PubMed29969945

2018

Specific autophagy and ESCRT components participate in the unconventional secretion of CFTR.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Hye Noh,Heon Yung Gee,Yonjung Kim,He Piao,Jiyoon Kim,Chung Min Kang,Gahyung Lee,Inhee Mook-Jung,Yangsin Lee,Jin Won Cho,Min Goo Lee

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Anti-GORASP2/GRASP55抗体

Anti-GORASP2/GRASP55 antibody

Western blot - Anti-GORASP2/GRASP55 antibody (AB74579)