Anti-GOLPH3/MIDAS抗体(ab69171)
Key features and details
- Mouse polyclonal to GOLPH3/MIDAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-GOLPH3/MIDAS抗体
参阅全部 GOLPH3/MIDAS 一抗 -
描述
小鼠多克隆抗体to GOLPH3/MIDAS -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human GOLPH3/MIDAS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_071413 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
靶标
-
功能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
组织特异性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
序列相似性
Belongs to the GOLPH3/VPS74 family. -
翻译后修饰
Phosphorylated. -
细胞定位
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
-
数据库链接
- Entrez Gene: 64083 Human
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- Unigene: 408909 Human
-
别名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
图片
-
All lanes : Anti-GOLPH3/MIDAS antibody (ab69171) at 1/500 dilution
Lane 1 : GOLPH3/MIDAS transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
-
Datasheet download
文献 (4)
ab69171 被引用在 4 文献中.
- Wang K et al. GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:575358 (2020). PubMed: 33134174
- Chen S et al. Widespread and Functional RNA Circularization in Localized Prostate Cancer. Cell 176:831-843.e22 (2019). PubMed: 30735634
- Li H et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer. J Transl Med 10:168 (2012). WB . PubMed: 22905766
- Wang JH et al. High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis. PLoS One 7:e45622 (2012). WB, IHC . PubMed: 23056210