AB69171

Anti-GOLPH3/MIDAS 抗体

Name: GOLPH3/MIDAS抗体 (ab69171)| Abcam中文官网
Brand: Abcam Limited
SKU: AB69171
Price: 4774 CNY
Availability: InStock
Rating: 5 (5 reviews)

Anti-GOLPH3/MIDAS antibody

(5 Reviews)

(5 Publications)

Mouse Polyclonal GOLPH3/MIDAS antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GOLPH3.

查看别名

GPP34, GOLPH3, Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS

1 Images

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Western blot - Anti-GOLPH3/MIDAS antibody (AB69171)

All lanes:

Western blot - Anti-GOLPH3/MIDAS antibody (ab69171) at 1/500 dilution

Lane 1:

GOLPH3/MIDAS transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 34 kDa

Observed band size: 30 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human GOLPH3.

Q9H4A6

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH3 also known as MIDAS (Mitochondrial Dynamics Associated) is a protein with a molecular weight of approximately 34 kDa. It is highly expressed in the Golgi apparatus a critical organelle for processing and sorting cellular proteins. GOLPH3 interacts with other proteins and lipids within the Golgi facilitating vesicle budding which is necessary for protein trafficking and secretion. The structural features of GOLPH3 allow it to bind phosphoinositides which indicates its role as a phosphatidylinositol 4-phosphate effector in the Golgi.

Biological function summary

GOLPH3/MIDAS plays a central role in maintaining Golgi structure and functionality. Its involvement in protein trafficking stems from its interaction with the cytoskeleton and Golgi membranes. GOLPH3 is part of a complex that includes several other proteins enhancing its ability to regulate Golgi shape and size which is essential for efficient cellular protein handling. The complex formation allows GOLPH3 to dynamically influence Golgi positioning within the cell an activity important for many cellular processes.

Pathways

The protein GOLPH3 interacts in the mTOR signaling and glycosylation pathways. The protein is associated with the regulation of mTOR signaling through its interaction with proteins like PIK3CA and AKT1. Additionally its role in glycosylation supports protein maturation and trafficking. These pathways are fundamental in cellular growth proliferation and survival and GOLPH3’s involvement highlights its multifunctional nature in cellular homeostasis.

GOLPH3 has connections to cancer and Alzheimer's disease. Its overexpression is linked to tumor development and progression in some cancers as it influences cell proliferation and survival through the mTOR pathway. In Alzheimer's disease abnormal Golgi structure and function have been noted and the dysregulation of GOLPH3 may contribute to the pathology. The association with proteins like tau and amyloid-beta in Alzheimer's further indicates the potential impact of GOLPH3 misregulation on disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

See full target information GOLPH3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:575358 PubMed33134174

2020

GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kebing Wang,Shuai Jiang,Anpei Huang,Ying Gao,Baogang Peng,Zhi Li,Wenbin Ma,Zhou Songyang,Shihong Zhang,Meifang He,Wen Li

Cell reports 30:3821-3836.e13 PubMed32187552

2020

APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Uzureau,Laurence Lecordier,Pierrick Uzureau,Dorle Hennig,Jonas H Graversen,Fabrice Homblé,Pepe Ekulu Mfutu,Fanny Oliveira Arcolino,Ana Raquel Ramos,Rita M La Rovere,Tomas Luyten,Marjorie Vermeersch,Patricia Tebabi,Marc Dieu,Bart Cuypers,Stijn Deborggraeve,Marion Rabant,Christophe Legendre,Søren K Moestrup,Elena Levtchenko,Geert Bultynck,Christophe Erneux,David Pérez-Morga,Etienne Pays

Cell 176:831-843.e22 PubMed30735634

2019

Widespread and Functional RNA Circularization in Localized Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sujun Chen,Vincent Huang,Xin Xu,Julie Livingstone,Fraser Soares,Jouhyun Jeon,Yong Zeng,Junjie Tony Hua,Jessica Petricca,Haiyang Guo,Miranda Wang,Fouad Yousif,Yuzhe Zhang,Nilgun Donmez,Musaddeque Ahmed,Stas Volik,Anna Lapuk,Melvin L K Chua,Lawrence E Heisler,Adrien Foucal,Natalie S Fox,Michael Fraser,Vinayak Bhandari,Yu-Jia Shiah,Jiansheng Guan,Jixi Li,Michèle Orain,Valérie Picard,Hélène Hovington,Alain Bergeron,Louis Lacombe,Yves Fradet,Bernard Têtu,Stanley Liu,Felix Feng,Xue Wu,Yang W Shao,Malgorzata A Komor,Cenk Sahinalp,Colin Collins,Youri Hoogstrate,Mark de Jong,Remond J A Fijneman,Teng Fei,Guido Jenster,Theodorus van der Kwast,Robert G Bristow,Paul C Boutros,Housheng Hansen He

PloS one 7:e45622 PubMed23056210

2012

High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Jian-Hua Wang,Xiu-Ting Chen,Zhe-Sheng Wen,Min Zheng,Jian-Ming Deng,Ming-Zhi Wang,Huan-Xin Lin,Kun Chen,Jun Li,Jing-Ping Yun,Rong-Zhen Luo,Li-Bing Song

Journal of translational medicine 10:168 PubMed22905766

2012

GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer.

Applications

Species

Unspecified reactive species

Huan Li,Ling Guo,Shu-Wei Chen,Xiao-Hui Zhao,Shi-Min Zhuang,Li-Ping Wang,Li-Bing Song,Ming Song

View all publications

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Anti-GOLPH3/MIDAS 抗体

Anti-GOLPH3/MIDAS antibody

Western blot - Anti-GOLPH3/MIDAS antibody (AB69171)