Anti-GNAT1抗体(ab3504)
Key features and details
- Rabbit polyclonal to GNAT1
- Suitable for: WB
- Reacts with: Sheep
- Isotype: IgG
概述
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产品名称
Anti-GNAT1抗体
参阅全部 GNAT1 一抗 -
描述
兔多克隆抗体to GNAT1 -
宿主
Rabbit -
特异性
Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Sheep
预测可用于: Rat, Dog, Human, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human GNAT1 aa 1-100.
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阳性对照
- WB: sheep retinal extracts
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). |
靶标
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功能
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
组织特异性
Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium. -
疾病相关
Night blindness, congenital stationary, autosomal dominant 3
Night blindness, congenital stationary, 1C -
序列相似性
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
发展阶段
Expressed at approximately postnatal day 7. - Information by UniProt
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数据库链接
- Entrez Gene: 403613 Dog
- Entrez Gene: 2779 Human
- Entrez Gene: 363143 Rat
- Entrez Gene: 399262 Xenopus laevis
- Omim: 139330 Human
- SwissProt: Q28300 Dog
- SwissProt: P11488 Human
- SwissProt: P38407 Xenopus laevis
see all -
别名
- CSNBAD3 antibody
- GBT1 antibody
- GNAT1 antibody
see all
图片
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Western blot - Anti-GNAT1 antibody (ab3504)
Western blot detection of Trabsducin alpha on sheep retinal extracts using ab3504.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab3504 被引用在 3 文献中.
- Menad R et al. G protein-coupled oestrogen receptor 1, oestrogen receptors and androgen receptor in the sand rat (Psammomys obesus) efferent ducts. Folia Morphol (Warsz) 79:756-766 (2020). PubMed: 32459366
- Taniuchi K et al. Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth. Oncogene 30:4843-54 (2011). PubMed: 21625220
- Adamus G et al. Molecular biomarkers for autoimmune retinopathies: significance of anti-transducin-alpha autoantibodies. Exp Mol Pathol 87:195-203 (2009). PubMed: 19744478