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Anti-GNAQ抗体(ab113429)

Submit a review Q&A (3)References (1)
Western blot - Anti-GNAQ antibody (ab113429)
  • Western blot - Anti-GNAQ antibody (ab113429)
  • Western blot - Anti-GNAQ antibody (ab113429)

Key features and details

  • Goat polyclonal to GNAQ
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

概述

  • 产品名称

    Anti-GNAQ抗体
    参阅全部 GNAQ 一抗

  • 描述

    山羊多克隆抗体to GNAQ

  • 宿主

    Goat

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, Pig
    预测可用于: Horse, Cow, Dog, Chinese hamster

  • 免疫原

    Synthetic peptide corresponding to Human GNAQ aa 150-250 (internal sequence) (Cysteine residue).
    Database link: P50148

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Human, mouse and rat testis tissue lysates, peripheral blood lymphocytes lysate and pig colon and spleen tissue lysates. IHC-P: Human kidney tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab113429于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 42 kDa). Primary incubation time: 1 hour.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 42 kDa). Primary incubation time: 1 hour.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Western blot - Anti-GNAQ antibody (ab113429)
    Western blot - Anti-GNAQ antibody (ab113429)
    All lanes : Anti-GNAQ antibody (ab113429) at 0.1 µg/ml

    Lane 1 : Mouse testis tissue lysate in RIPA buffer
    Lane 2 : Rat testis tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

  • Western blot - Anti-GNAQ antibody (ab113429)
    Western blot - Anti-GNAQ antibody (ab113429)
    Anti-GNAQ antibody (ab113429) at 0.1 µg/ml + Peripheral blood lymphocyte lysate in RIPA buffer at 35 µg

    Predicted band size: 42 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

  • Western blot - Anti-GNAQ antibody (ab113429)
    Western blot - Anti-GNAQ antibody (ab113429)
    All lanes : Anti-GNAQ antibody (ab113429) at 0.1 µg/ml

    Lane 1 : Pig colon tissue lysate in RIPA buffer
    Lane 2 : Pig spleen tissue lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

文献 (1)

发表研究结果有使用 ab113429？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab113429 被引用在 1 文献中.

  • Li Y  et al. The oncolytic virus H101 combined with GNAQ siRNA-mediated knockdown reduces uveal melanoma cell viability. J Cell Biochem 120:5766-5776 (2019). PubMed: 30320917

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

I have decided on a replacement antibody - could you please send me - ab113966, an anti-KCNK5 antibody. Please let me know if there are additional charges for delivery etc as we will need to raise a PO to cover this.



many thanks - Steve

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement at absolutely no cost for you with the order number 123456.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

Many thanks for getting back to me, and offering to replace the antibody. Unfortunately you do not have another antibody that is suitable for this study (i.e. a goat antibody recognising both Gnaq and Gna11) - i will therefore select another antibody - but at the moment i am not sure what this will be. it will take me a day or two to sort this out and see what else could be useful. I will let you know when i have decided



I will also add my characterisation data to the abreview for the goat Gnaq antibody - as i say it does work quite well - but only for Gnaq.



Thanks again

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Answer

Thank you for your reply.



Please ne assured that there is no rush to pick a new antibody "just because". If you need a week or two to decide, than this is absolutely fine. In case you are not sure that we have an antibody you need right now, I am happy to issue you a credit note which can be used later.

I hope this information is helpful to you. Please let me know what you prefer.


Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I have recently purchased antibody ab113429 - advertised as a Gnaq/11 antibody (i.e. will recognise both Gnaq and Gna11 - not suprising as these proteins are highly related). However, this antibody recognises only Gnaq and not Gna11 as indicated on the data sheet. A simple blast search confirms that this antibody will not recognise Gna11 - and i have also confirmed this by labeling of cells transfected with Gnaq and Gna11 etc. Now that i have checked more fully it would seem that the antigen is actually the same as that used to raise another one of your antibodies - ab128060 - which is clearly stated to be Gnaq specific. The issue is that i am using ab113429 to confirm the pattern of staining observed with another Gnaq/11 antibody (ab79337) - which labels both Gnaq and Gna11 (i have confirmed the specificity of this antibody using transfected cells etc) - ab113429 is also raised in goat and i had planned to use this for double labeling with other rabbit antibodies. The problem is that as the new antibody does not recognise Gnaq/11 as stated, but only Gnaq - it is of little use to me as a control or for double labeling. (all antibodies do seem to work quite well - its just that ab113429 fails to detect Gna11 as implied by the data sheet / antibody name. On the basis that the antibody is not as expected i would a refund or to replace it with a different one? is this possible? I am more than willing to send you the characterisation data if you require.

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Answer

Thank you for contacting us and bringing this to our attention.

I can confirm that the identity is way too low to react with the G protein alpha 11 protein at 61%. I will gladly issue you a replacement for free of your choice. I have also arranged that the name will be updated.

In addition, we will contact our other customers to inform them that this antibody is not suitable to detect these two proteins at the same time.


Nonetheless, we would be very happy to receive the data about your experiments in form of an Abreview, so we can share the information with the rest of our customers. We would reward you with our loyalty points which can be used for further orders. https://www.abcam.com/abreviews


I am looking forward to hearing back from you.

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