AB317136

Anti-GNAI3 antibody [HL2096]

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Rabbit Monoclonal GNAI3 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3.

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, GNAI3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody [HL2096] (AB317136)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody [HL2096] (AB317136)

ab317136 detects GNAI3 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. GNAI3 stained by ab317136 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody [HL2096] (AB317136)

ab317136 detects GNAI3 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. GNAI3 stained by ab317136 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-GNAI3 antibody [HL2096] (AB317136)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317136 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNAI3 antibody [HL2096] (ab317136) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-GNAI3 antibody [HL2096] (AB317136)

Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317136 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNAI3 antibody [HL2096] (ab317136) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

GNAI3 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-GNAI3 antibody [HL2096] (AB317136)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317136 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GNAI3 antibody [HL2096] (ab317136) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-GNAI3 antibody [HL2096] (AB317136)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317136 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GNAI3 antibody [HL2096] (ab317136) at 1/5000 dilution

Lane 1:

Non-transfected (–) whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged GNAI1 transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

DDDDK-tagged GNAI2 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged GNAI3 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL2096

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human Guanine nucleotide-binding protein G(i) subunit alpha-3.

P08754

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GNAI3 also known as the guanine nucleotide-binding protein G(i) alpha-3 subunit is part of the heterotrimeric G protein family. Its molecular weight is approximately 41 kDa. This protein plays a role in transmitting signals from extracellular stimuli through cell-surface receptors to the intracellular signaling pathways. GNAI3 is expressed in various tissues including the brain and heart where it facilitates important cellular responses by interacting with other proteins in signaling pathways.

Biological function summary

GNAI3 contributes to inhibiting adenylate cyclase activity reducing cyclic AMP levels and therefore modulating downstream signaling events. It forms part of the heterotrimeric guanine nucleotide-binding protein complex which consists of alpha beta and gamma subunits. By interacting with this complex GNAI3 regulates processes like cell division immune response and sensory perception demonstrating its diverse functional roles within the cell.

Pathways

GNAI3 is involved in the G protein-coupled receptor (GPCR) signaling pathway which is essential for transmitting numerous extracellular signals to the inside of the cell. GNAI3 modulates signals from neurotransmitters and hormones that activate this pathway. Additionally GNAI3 interacts with other proteins such as beta-arrestins which are important for receptor desensitization and signal termination within the GPCR signaling system.

Researchers have linked GNAI3 disruptions to auriculocondylar syndrome and certain forms of cancer. In auriculocondylar syndrome mutations in GNAI3 contribute to craniofacial abnormalities. Additionally alterations in its signaling pathways might contribute to tumor progression by failing to regulate cell proliferation properly. GNAI3 interacts with proteins including RGS proteins which help in temporally regulating signal transduction showing its significance in health and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 19478087, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed : 19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed : 2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed : 17635935).

See full target information Guanine nucleotide-binding protein G(i) subunit alpha-3

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Anti-GNAI3 antibody [HL2096]