AB249765

重组Anti-GNAI3抗体[EPR11894] - BSA and Azide free

Anti-GNAI3 antibody [EPR11894] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GNAI3 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

查看别名

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, GNAI3

2 Images

Supplier Data

Immunoprecipitation - Anti-GNAI3 antibody [EPR11894] - BSA and Azide free (AB249765)

This data was developed using ab173527, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab173527 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-GNAI3 antibody [EPR11894] (<a href='/products/primary-antibodies/gnai3-antibody-epr11894-ab173527'>ab173527</a>)

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-GNAI3 antibody [EPR11894] - BSA and Azide free (AB249765)

This data was developed using ab173527, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GNAI3 antibody [EPR11894] (<a href='/products/primary-antibodies/gnai3-antibody-epr11894-ab173527'>ab173527</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Lane 3:

HepG2 lysates at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 41 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-GNAI3 antibody [EPR11894]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11894

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The peptide immunogen shares a 92.9% homology with GNAI1 and GNAI2, therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.

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反应性数据

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产品详情

ab249765 is the carrier-free version of ab173527.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GNAI3 also known as the guanine nucleotide-binding protein G(i) alpha-3 subunit is part of the heterotrimeric G protein family. Its molecular weight is approximately 41 kDa. This protein plays a role in transmitting signals from extracellular stimuli through cell-surface receptors to the intracellular signaling pathways. GNAI3 is expressed in various tissues including the brain and heart where it facilitates important cellular responses by interacting with other proteins in signaling pathways.

Biological function summary

GNAI3 contributes to inhibiting adenylate cyclase activity reducing cyclic AMP levels and therefore modulating downstream signaling events. It forms part of the heterotrimeric guanine nucleotide-binding protein complex which consists of alpha beta and gamma subunits. By interacting with this complex GNAI3 regulates processes like cell division immune response and sensory perception demonstrating its diverse functional roles within the cell.

Pathways

GNAI3 is involved in the G protein-coupled receptor (GPCR) signaling pathway which is essential for transmitting numerous extracellular signals to the inside of the cell. GNAI3 modulates signals from neurotransmitters and hormones that activate this pathway. Additionally GNAI3 interacts with other proteins such as beta-arrestins which are important for receptor desensitization and signal termination within the GPCR signaling system.

Researchers have linked GNAI3 disruptions to auriculocondylar syndrome and certain forms of cancer. In auriculocondylar syndrome mutations in GNAI3 contribute to craniofacial abnormalities. Additionally alterations in its signaling pathways might contribute to tumor progression by failing to regulate cell proliferation properly. GNAI3 interacts with proteins including RGS proteins which help in temporally regulating signal transduction showing its significance in health and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 18434541, PubMed : 19478087, PubMed : 8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed : 19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed : 2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed : 17635935). The active GTP-bound form activates the calcium permeant TRPC5 ion channels (PubMed : 37137991).

See full target information Guanine nucleotide-binding protein G(i) subunit alpha-3

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-GNAI3抗体[EPR11894] - BSA and Azide free