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重组RabMAb

重组Anti-GNAI3抗体[EPR11894] (ab173527)

Reviews (1) Submit a question References (3)
Western blot - Anti-GNAI3 antibody [EPR11894] (ab173527)
  • Immunoprecipitation - Anti-GNAI3 antibody [EPR11894] (ab173527)
  • Anti-GNAI3 antibody [EPR11894] (ab173527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11894] to GNAI3
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-GNAI3抗体[EPR11894]
    参阅全部 GNAI3 一抗

  • 描述

    兔单克隆抗体[EPR11894] to GNAI3

  • 宿主

    Rabbit

  • 特异性

    The peptide immunogen shares a 92.9% homology with GNAI1 and GNAI2, therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.

  • 经测试应用

    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Xenopus laevis

  • 免疫原

    Synthetic peptide within Human GNAI3 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P08754

  • 阳性对照

    • WB: Human fetal brain, fetal kidney, HepG2 and A431 lysates. Immunoprecipitation pellet from Human fetal brain lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR11894

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab173527于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP
1/10 - 1/100.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP
1/10 - 1/100.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

靶标

  • 功能

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

  • 序列相似性

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.

  • 细胞定位

    Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 87U6 antibody
    • FLJ26559 antibody
    • G protein alpha inhibiting 3 antibody
    • G(i) alpha 3 antibody
    • G(i) alpha-3 antibody
    • GNAI3 antibody
    • GNAI3_HUMAN antibody
    • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody
    • Guanine nucleotide binding protein G(k) alpha subunit antibody
    • Guanine nucleotide-binding protein G(k) subunit alpha antibody
    • OTTHUMP00000013368 antibody
    see all

图片

  • Western blot - Anti-GNAI3 antibody [EPR11894] (ab173527)
    Western blot - Anti-GNAI3 antibody [EPR11894] (ab173527)
    All lanes : Anti-GNAI3 antibody [EPR11894] (ab173527) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal kidney lysates
    Lane 3 : HepG2 lysates
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Immunoprecipitation - Anti-GNAI3 antibody [EPR11894] (ab173527)
    Immunoprecipitation - Anti-GNAI3 antibody [EPR11894] (ab173527)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab173527 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-GNAI3 antibody [EPR11894] (ab173527)
    Anti-GNAI3 antibody [EPR11894] (ab173527)

文献 (3)

发表研究结果有使用 ab173527？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab173527 被引用在 3 文献中.

  • Liu X  et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
  • Nusková H  et al. Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins. Nat Commun 12:4590 (2021). PubMed: 34321466
  • Freeman S  et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879

客户评价及客户问答

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Western blot abreview for Anti-GNAI3 antibody [EPR11894]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Rat Purified protein (BL21 RIL competent cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris Gel)
Loading amount
0.5 µg
Specification
BL21 RIL competent cells
Blocking step
iBind Solution as blocking agent for 10 minute(s) · Concentration: 100%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Kevin Ebert

Verified customer

提交于 Sep 28 2018

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