Anti-GNAI1 + GNAI2抗体(ab3522)

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Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)

Key features and details

  • Rabbit polyclonal to GNAI1 + GNAI2
  • Suitable for: IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-GNAI1 + GNAI2抗体

  • 描述

    兔多克隆抗体to GNAI1 + GNAI2

  • 宿主

    Rabbit

  • 特异性

    Similarity with other sequences from the G protein family is predicted but has not being tested empirically. 

  • 经测试应用

    适用于: IHC-P, ICCmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish

  • 免疫原

    Synthetic peptide corresponding to Human GNAI1 + GNAI2 aa 300-400.
    Database link: P63096
    (Peptide available as ab5006)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-P: Human tonsil tissue. ICC: SH-SY5Y cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3522于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 2 µg/ml.
说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 2 µg/ml.

靶标

  • 功能

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.

  • 序列相似性

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.

  • 细胞定位

    Nucleus. Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Adenylate cyclase-inhibiting G alpha protein antibody
    • Gi antibody
    • Gi1 protein alpha subunit antibody
    • Gi2 protein alpha subunit antibody
    • GIP antibody
    • GNAI1 antibody
    • GNAI1_HUMAN antibody
    • GNAI2 antibody
    • GNAI2B antibody
    • Guanine nucleotide binding protein G(i) alpha 1 subunit antibody
    • Guanine nucleotide binding protein G(i) alpha 2 subunit antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-1 antibody
    see all

图片

  • Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)
    Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)

    SH-SY5Y cells stained for GNAI1 (green) using ab3522 at 2 µg/mL in immunocytochemical analysis. The nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI, F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel e shows the no primary antibody control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)

    IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (5)

发表研究结果有使用 ab3522？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3522 被引用在 5 文献中.

  • Pagano Zottola AC  et al. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells 11:N/A (2022). PubMed: 36010658
  • Vosahlikova M  et al. Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of d-opioid receptor amount and function. Biochem Pharmacol 154:452-463 (2018). PubMed: 29883706
  • Cechova K  et al. Up-regulation of µ-, d- and ?-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes. J Neuroimmunol 321:12-23 (2018). PubMed: 29957382
  • Doi M  et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). WB . PubMed: 26882873
  • Ufer C & Germack R Cross-regulation between beta1- and beta3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes. Br J Pharmacol 158:300-13 (2009). WB ; Rat . PubMed: 19719783

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