Anti-Glypican 4抗体(ab52509)
Key features and details
- Rabbit polyclonal to Glypican 4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glypican 4抗体
参阅全部 Glypican 4 一抗 -
描述
兔多克隆抗体to Glypican 4 -
宿主
Rabbit -
特异性
Specific for a core protein epitope (aa220-233) of human Glypican 4. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
TFAQGLAVAG-DVV
conjugated to KLH, corresponding to amino acids 220-233 of Human Glypican 4 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. -
序列相似性
Belongs to the glypican family. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 2239 Human
- Omim: 300168 Human
- SwissProt: O75487 Human
- Unigene: 58367 Human
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别名
- dJ900E8.1 glypican 4 antibody
- Glypican proteoglycan 4 antibody
- Gpc4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (ab52509)
IHC image of ab52509 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52509, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (2)
ab52509 被引用在 2 文献中.
- Zhang Y et al. Comparative Glycoproteomic Profiling of Human Body Fluid between Healthy Controls and Patients with Papillary Thyroid Carcinoma. J Proteome Res 19:2539-2552 (2020). PubMed: 31800250
- Iacobas S et al. The connexin43-dependent transcriptome during brain development: Importance of genetic background. Brain Res : (2012). PubMed: 22771707
