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重组RabMAb

重组Anti-Glypican 1/ GPC1抗体[EPR19285] - BSA and Azide free (ab271962)

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Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
  • Flow Cytometry (Intracellular) - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
  • Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 APC PE Unconjugated

概述

  • 产品名称

    Anti-Glypican 1/ GPC1抗体[EPR19285] - BSA and Azide free
    参阅全部 Glypican 1/ GPC1 一抗

  • 描述

    兔单克隆抗体[EPR19285] to Glypican 1/ GPC1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt (intra): MDA-MB-231 cells.

  • 常规说明

    ab271962 is the carrier-free version of ab199343.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271962于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Cell surface proteoglycan that bears heparan sulfate.

  • 序列相似性

    Belongs to the glypican family.

  • 翻译后修饰

    This cell-associated glypican is further processed to give rise to a medium-released species.

  • 细胞定位

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • FLJ38078 antibody
      • glypican antibody
      • Glypican proteoglycan 1 antibody
      • glypican1 antibody
      • GPC 1 antibody
      • Gpc1 antibody
      • GPC1_HUMAN antibody
      • Secreted glypican-1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
      Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343).
    • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
      Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining on MCF7 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343).
    • Flow Cytometry (Intracellular) - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
      Flow Cytometry (Intracellular) - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343). 

       

       

    • Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
      Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271962？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271962 尚未被引用在任何文献中。

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