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Anti-Glypican 1/ GPC1抗体(ab55971)

Submit a review Q&A (2)References (8)

Key features and details

  • Rabbit polyclonal to Glypican 1/ GPC1
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (ab255600)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Glypican 1/ GPC1抗体
    参阅全部 Glypican 1/ GPC1 一抗

  • 描述

    兔多克隆抗体to Glypican 1/ GPC1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide (Human) corresponding to a C-terminal sequence.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab55971于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

文献 (8)

发表研究结果有使用 ab55971？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab55971 被引用在 8 文献中.

  • Chen G  et al. High glypican-1 expression is a prognostic factor for predicting a poor clinical prognosis in patients with hepatocellular carcinoma. Oncol Lett 20:197 (2020). PubMed: 32963603
  • Kölbel H  et al. Identification of Candidate Protein Markers in Skeletal Muscle of Laminin-211-Deficient CMD Type 1A-Patients. Front Neurol 10:470 (2019). PubMed: 31133972
  • Qadir F  et al. Transcriptome reprogramming by cancer exosomes: identification of novel molecular targets in matrix and immune modulation. Mol Cancer 17:97 (2018). PubMed: 30008265
  • Cave C  et al. GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord. Mol Neurodegener 12:8 (2017). PubMed: 28103900
  • Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015). WB . PubMed: 26403541
  • Micieli JA  et al. Anti-glycophorin C induces mitochondrial membrane depolarization and a loss of extracellular regulated kinase 1/2 protein kinase activity that is prevented by pretreatment with cytochalasin D: implications for hemolytic disease of the fetus and newborn caused by anti-Ge3. Transfusion 50:1761-5 (2010). PubMed: 20456692
  • Taylor DR  et al. Glypican-1 mediates both prion protein lipid raft association and disease isoform formation. PLoS Pathog 5:e1000666 (2009). WB ; Mouse . PubMed: 19936054
  • Elschenbroich S  et al. Peptide separations by on-line MudPIT compared to isoelectric focusing in an off-gel format: application to a membrane-enriched fraction from C2C12 mouse skeletal muscle cells. J Proteome Res 8:4860-9 (2009). PubMed: 19670906

客户评价及客户问答

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Question

please I contacted the customer and she prefers to get a refund. Please proceed with a credit note.

Thanks in advance
Kind regards

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Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: ####


As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Question

LOT NUMBER GR47361-2

ORDER NUMBER 3294

DESCRIPTION OF THE PROBLEM no signal

SAMPLE cell extract from SCn2a scrapie-infected neuroblastoma N2a cells murine HeLa, 293T Human Embryonic Kidney

PRIMARY ANTIBODY 1:500 and 1:200, TBST 1% BSA o/n 4C

DETECTION METHOD ECL ultra Pierce

POSITIVE AND NEGATIVE CONTROLS USED SCn2a HeLa, 293T

ANTIBODY STORAGE CONDITIONS aliquoted and storaged at -20C

SAMPLE PREPARATION buffer NP40+ protease inhibitors

AMOUNT OF PROTEIN LOADED 30γ

ELECTROPHORESIS/GEL CONDITIONS 10% gel, electophoresis with Beta-mercaptoethanol

TRANSFER AND BLOCKING CONDITIONS 2h, 400mA, Biorad system, transfer buffer Tris/Glycine/Methanol, Blocking reagent: no fat dry milk 5%

SECONDARY ANTIBODY rabbit Amersham 1:4000 milk 5% 1h, 4 washes , 5 min/each in TBS tween 0,2%

HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3

HAVE YOU RUN A NO PRIMARY CONTROL? No

DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? none

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably recieved a bad vial.

I apologise for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation.

In addition to this, I can suggest you may like to consider trying the secondary antibody with a different primary antibody to ensure the current vial is working if this has not already been tried.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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