Anti-Glypican 1/ GPC1抗体(ab106003)
Key features and details
- Rabbit polyclonal to Glypican 1/ GPC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glypican 1/ GPC1抗体
参阅全部 Glypican 1/ GPC1 一抗 -
描述
兔多克隆抗体to Glypican 1/ GPC1 -
宿主
Rabbit -
特异性
A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Macaque monkey, Opossum
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免疫原
A synthetic peptide corresponding to an internal region of Human Glypican 1/ GPC1
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Anti-Glypican-1 was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 62 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 62 kDa. |
靶标
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功能
Cell surface proteoglycan that bears heparan sulfate. -
序列相似性
Belongs to the glypican family. -
翻译后修饰
This cell-associated glypican is further processed to give rise to a medium-released species. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 518114 Cow
- Entrez Gene: 2817 Human
- Omim: 600395 Human
- SwissProt: G3X745 Cow
- SwissProt: P35052 Human
- Unigene: 328232 Human
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别名
- FLJ38078 antibody
- glypican antibody
- Glypican proteoglycan 1 antibody
see all
图片
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Western blot - Anti-Glypican 1/ GPC1 antibody (ab106003)All lanes : Anti-Glypican 1/ GPC1 antibody (ab106003) at 1/1000 dilution
Lane 1 : Untransfected 293T whole cell lysate
Lane 2 : Glypican 1/ GPC1 transfected 293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP Goat-anti-Rabbit IgG at 1/5000 dilution
Predicted band size: 62 kDa
The higher molecular weight (110kDa) of transfected Glypican 1/ GPC1 compared with the expected MW is likely due to the presence of an Fc-tag.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab106003 被引用在 2 文献中.
- Gao P et al. Preventive effect on endothelial surface layer damage of Fusu agent in LPS-induced acute lung injury in rats. Mol Cell Biochem N/A:N/A (2018). PubMed: 29948507
- Li C et al. GPC1 regulated by miR-96-5p, rather than miR-182-5p, in inhibition of pancreatic carcinoma cell proliferation. Int J Mol Sci 15:6314-27 (2014). WB ; Human . PubMed: 24736782