Anti-Glycophorin A抗体(ab40844)
Key features and details
- Goat polyclonal to Glycophorin A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glycophorin A抗体
参阅全部 Glycophorin A 一抗 -
描述
山羊多克隆抗体to Glycophorin A -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
SVEIENPETSDQ
, corresponding to C terminal amino acids 139-150 of Human Glycophorin A -
阳性对照
- WB: Human peripheral blood monocytes and kidney lysates. Recombinant Human Glycophorin A protein (ab114330).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa).
1 hour primary incubation is recommended for this product. The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624). |
说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 16 kDa). 1 hour primary incubation is recommended for this product. The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624). |
靶标
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功能
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
序列相似性
Belongs to the glycophorin A family. -
翻译后修饰
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
细胞定位
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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数据库链接
- Entrez Gene: 2993 Human
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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别名
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
图片
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All lanes : Anti-Glycophorin A antibody (ab40844) at 1 µg/ml
Lane 1 : Human peripheral blood monocyte lysate
Lane 2 : Human kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 16 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
The observed molecular weight differs from the predicted molecular weight due to the formation of dimers as described in the literature (PMID: 8204624).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab40844 被引用在 1 文献中.
- Michels KR et al. Preclinical proof of concept for VivoVec, a lentiviral-based platform for in vivo CAR T-cell engineering. J Immunother Cancer 11:N/A (2023). PubMed: 36918221