重组Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体[EP852Y] (ab81230)
![Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)](https://www.abcam.cn/ps/products/81/ab81230/Images/ab81230-273212-anti-glycogen-synthase-1-phospho-s641-antibody-ep852y-western-blot.jpg "Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP852Y] to Glycogen synthase 1/GYS1 (phospho S641)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体[EP852Y]
参阅全部 Glycogen synthase 1/GYS1 一抗 -
描述
兔单克隆抗体[EP852Y] to Glycogen synthase 1/GYS1 (phospho S641) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse muscle and Mouse muscle treated with AP lysates; HeLa and HeLa treated with insulin at 20 ug/mL for 15 minutes whole cell lysates. IHC: Human liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP852Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81230于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 85 kDa.
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Dot blot
1/1000. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 85 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt or ICC/IF.
靶标
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功能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
通路
Glycan biosynthesis; glycogen biosynthesis. -
疾病相关
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
序列相似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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数据库链接
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- Unigene: 386225 Human
- Unigene: 275654 Mouse
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别名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
图片
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Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysates
Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 1 secondBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)](https://www.abcam.cn/ps/products/81/ab81230/Images/ab81230-96404-anti-glycogen-synthase-1-phospho-s641-antibody-ep852y-western-blot.jpg)
Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilution
Lane 1 : Mouse muscle tissue lysate, untreated
Lane 2 : Mouse muscle tissue lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
![Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)](https://www.abcam.cn/ps/products/81/ab81230/Images/ab81230-271118-anti-glycogen-synthase-1-phospho-s641-antibody-ep852y-dot-blot.jpg)
Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)Dot blot analysis of glycogen synthase 1/GYS1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1/GYS1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)](https://www.abcam.cn/ps/products/81/ab81230/Images/ab81230-96403-anti-glycogen-synthase-1-phospho-s641-antibody-ep852y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)](https://www.abcam.cn/ps/products/81/ab81230/Images/ab81230-6-benefits-of-recombinant-antibodies.png)
Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab81230 被引用在 11 文献中.
- Rozhkov SV et al. The Role of Glycogen Synthase Kinase-3 in the Regulation of Ribosome Biogenesis in Rat Soleus Muscle under Disuse Conditions. Int J Mol Sci 23:N/A (2022). PubMed: 35269893
- Hu MM et al. Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food Nutr Res 66:N/A (2022). PubMed: 35382382
- Sugiura T et al. Screening of a Panel of Low Molecular Weight Compounds That Inhibit Synovial Fibroblast Invasion in Rheumatoid Arthritis. J Immunol 205:3277-3290 (2020). PubMed: 33177160
- Shen J et al. Pressure suppresses hepatocellular glycogen synthesis through activating the p53/Pten pathway. Mol Med Rep 19:5105-5114 (2019). PubMed: 31059076
- Barker RG et al. Elevated GLUT4 and glycogenin protein abundance correspond to increased glycogen content in the soleus muscle of mdx mice with no benefit associated with taurine supplementation. Physiol Rep 6:N/A (2018). PubMed: 29484837